中文摘要：

目的探讨河蚌多糖（AP）体外抗乙肝病毒（HBV）的生物活性及量效关系。方法以Hep G2.2.15细胞为体外细胞模型,与1：10梯度稀释的AP 37℃孵育9d,ELISA法检测细胞上清中HBsAg、HBeAg的含量,荧光定量PCR检测HBV DNA载量。采用Thiele型连分式插值法检测AP半数抑制浓度（IC50）、最大抑制率并绘制量效曲线图。结果 AP在体外可显著抑制Hep G2.2.15细胞的HBsAg和HBeAg分泌以及HBV DNA复制,且呈现剂量依赖关系。通过Thiele型连分式插值法得出量效关系表达式,并由此确定AP对HBeAg和HBsAg分泌的最大抑制率分别为47.7%和56.4%（抑制HBeAg的IC50为143.7mg/L）;对HBV DNA复制的最大抑制率为17.8%。结论 AP具有体外抗HBV生物活性。Thiele型连分式插值法简单、实用,是一种分析药物活性的新方法。

英文摘要：

Objective To explore the anti-HBV activity of anodonta polysaccharides（AP） and dose-effect relationship in vitro. Methods Hep G2.2.15 cells were cultured in vitro and incubated at 37℃ for nine days with AP at a dilution ratio of 1：10. The expression of HBsAg and HBeAg were detected using ELISA and HBV-DNA copies were detected by real-time fluorescent quantitative PCR. Based on Thiele-type continued-fraction interpolation method, the anti-HBV activity of AP was studied, and the IC50 and the maximum inhibition rate were calculated. Results AP had significant inhibitory effect on the expression of HBsAg and HBeAg in Hep G2.2.15 cells in vitro, as well as HBV DNA replication. By Thiele-type continued-fraction interpolation the equations of dose-effect relationship were obtained to determine the maximum inhibition rates of AP on HBeAg and HBsAg secretion being 47.7% and 56.4%, and the IC50 inhibiting the expression of HBeAg being 143.7mg/L. AP was also able to inhibit HBV-DNA replication and the maximum inhibition rate was 17.8% with the same method above. Conclusion Anodonta polysaccharides have anti-HBV activity. The thiele-type continued-fraction interpolation method is simple and practical and could be used as a new method for the analysis of drug activity.