中文摘要：

目的：体外研究药对防风-乌梅抑制肥大细胞脱颗粒的作用及新机制,为该药在临床治疗过敏性疾病提供科学依据。方法：体外采用胰蛋白酶刺激后P815细胞方法建立肥大细胞脱颗粒模型,采用RT-PCR、Western blotting、ELISA等,观察药对防风-乌梅对肥大细胞分泌组胺,PARs、PAR-2表达的影响。结果：药对防风-乌梅在体外可抑制肥大细胞分泌组胺及PARs、PAR-2表达,与细胞模型组比较,差异有统计学意义（P〈0.05,P〈0.01）。结论：药对防风-乌梅可能通过调节PAR-2表达阻断肥大细胞脱颗粒,抑制肥大细胞＂瀑布效应＂,从而达到抗过敏作用。

英文摘要：

Objective： To study the new mechanism and effect of medicine suits as divaricate saposhnikovia rootsmoked plum on expression of PAR-2 and blocking mast cell degranulation, in order to provide a scientific basis for clinical application of this medicine suits in the treatment of allergic diseases. Methods： Mast cell degranulation model was established by stimulating P815 cell with trypsin in vitro, and then the expressions of PARs and PAR-2 in mast cells and the levels of histamine affected by divaricate saposhnikovia root-smoked plum were observed by using RT-PCR, Western blotting and ELISA. Results： Medicine suits as divaricate saposhnikovia root-smoked plum could inhibit the expression of PARs and PAR-2, and the secretion of histamine in mast cells significantly in vitro, and there was a statistically significance compared with model group（P0.05, P0.01）. Conclusion： Medicine suits as divaricate saposhnikovia root-smoked plum could play a role of anti-anaphylactic action through regulating the expression of PAR-2 to block mast cell degranulation, and suppress the waterfall effect of mast cells.