中文摘要：

目的研究阳离子脂质体为载体的寡核苷酸(oligodeoxynucleotides,ODNs)转染原代培养的大鼠腹腔巨噬细胞(pMφ)的时相关系、分布以及转染效率。方法以阳离子脂质体为载体,将荧光标记的圈套ODNs(2.0μg/ml)转染入纯化的大鼠pMφ细胞,分别在30 min、1 h,3 h,6 h,9 h,12 h,18 h,24 h用荧光显微镜及激光共聚焦显微镜观察荧光标记的圈套ODNs在细胞内的动态变化。结果荧光显微镜下转染30 min细胞内便有荧光标记的圈套ODNs出现,转染6~12 h细胞内的荧光最强。激光共聚焦显微镜显示转染30min细胞内荧光标记的圈套ODNs主要分布在胞浆内,随着时间的延长,细胞内尤其胞核内的荧光越来越强,转染6~12h细胞内的荧光最强。Results:结论以阳离子脂质体为载体的哑铃形圈套ODNs可转染入大鼠pMφ细胞并分布于胞浆及胞核。

英文摘要：

Objective: To study the time,distribution and transfection efficiency of Decoy-ODNs transfection into the primarily cultured peritoneal macrophages(pMφ) cells using cationic liposomes as carriers.Methods: Using cationic liposomes as carriers,Decoy-ODNs(2.0 μg/ml) labeled with fluorescein was transfected into the purified pMφ cells from rats,and the dynamic intracellular changes of Decoy ODNs were observed at 30 minutes,1,3,6,9,12,18 and 24 hours after transfection by fluorescent microscope and confocal lase...