中文摘要：

的探讨tk基因系统在体外培养条件下对T细胞克隆的转导情况以及转导后的tk杀伤效应。方法将tk基因在体外培养条件下导入结肠抗原特异性T细胞克隆，观察基因转染、克隆凋亡情况，比较tk^＋和tk^-T细胞克隆之白细胞介素（IL）-2分泌水平、细胞存活率、“旁观者效应”间差异。结果荧光显微镜下观察可见转染成功，tk基因导入淋巴细胞发生凋亡；当复合感染度（MOI）为0、1．0、5．0、20．0、50．0、100．0、200．0、500．0时，细胞存活率分别为（94．43±5．02）％、（85．33±4．12）％、（81．07±2．46）％、（73．51±3．89）％、（68．68±5．26）％、（54．16±3．72）％、（45．51±2．29）％、（22．16±1．77）％。随着MOI和丙氧鸟苷（GCV）浓度增加，细胞存活率呈现明显下降趋势；tk^＋T细胞克隆实验组IL-2分泌水平明显下降，与对照组差异有统计学意义（P〈0．01）；各种tk^＋T细胞比例条件下的存活率变化曲线与ADV／Empty组接近，未能产生类似肿瘤细胞的旁观者效应。结论tk基因对T细胞克隆具备杀伤效应，但tk^＋T细胞克隆杀伤实验中未能见到与肿瘤细胞类似的“旁观者效应”。

英文摘要：

Objective To explore the killing effect of Hsv-tk gene induced into T lymphocyte clone ex vivo. Methods Hsv-tk suicide gene was induced into the T lymphocyte clone,then the change of gene transferred and the clone apoptosis were observed before and after induction. The difference in the level of IL-2 secretion in CTL or the livability of T lymphocyte or ＂Stander-by effect＂ were compared. Results The tk gene was induced successfully and the apoptosis was observed in the tk^＋ clone. When the MOI was 0,1.0,5.0,20.0,50.0,100.0,200.0 and 500.0,the livability of tk^＋ T lymphocyte was separately （94.43± 5.02）%, （85.33± 4.12）%, （81.07± 2.46）%, （73.51± 3.89）%, （68.68± 5.26）%,（54.16±3.72）%,（45.51±2.29）% and （22.16±1.77）%.When the MOI andGCVwere increased,the livability of tk^＋T lymphocyte was decreased significantly. The level of IL-2 secretion in experimental group was decreased significantly （P〈 0.01 ）. The livability change curve under the different proportion was close to ADV/Empty. The ＂Stander-by effect＂ was not found. Conclusion tk^＋ lymphocyte could produce killing effect. But the ＂Stander-by effect＂ was not found in tk^＋ lymphocyte and cell clone.