以狭鳕加工废料为来源提取酸溶胶原并加以表征。该胶原在230nm处有最大紫外吸收,红外吸收光谱指示存在螺旋结构。电泳图谱分离出多个亚基,鉴定为I型胶原。该胶原有独特的氨基酸组成,甘氨酸达19.8%,羟脯氨酸占6.9%。酸水解制备的胶原多肽平均分子量区间为3.2~53kDa。胶原多肽具有不同的生物学功能:较高分子量的胶原多肽用于小鼠表现出止血、促凝血和促进外伤愈合的功能,而较低分子量的胶原多肽则对烫伤修复有显著的促进作用。
Acid-soluble collagen from the processing waste of Alaska pollock was extracted and characterized.It had a maximum UV absorbance at 230nm while amino acid composition and SDS-PAGE suggested it was type I collagen.FTIR spectra showed the existence of helical arrangements.Hydrolysates were prepared by acid hydrolysis.The average molecular weights of its hydrolysates ranged from 3.2 to 53kDa.Functional properties of the collagen have been diversified by hydrolysis.For the collagen polypeptides of higher molecular weight,stimulatory effects on hemostasis and wound healing were found.Besides,clotting time reduction in mice was also revealed.While the molecules of lower molecular weight showed positive effects on scald repair.