中文摘要：

目的探讨具备有序或无序拓扑结构的聚甲基丙烯酸甲酯（polymethylmethacrylate,PMMA）电纺纳米纤维材料作为原代大鼠背根神经元（dorsal root ganglion neurons,DRGn）负载支架的可行性。方法构建具有随机或有序拓扑结构的PMMA电纺纳米纤维;利用PMMA薄膜组作为对照,分离纯化大鼠原代DRGn,利用慢病毒技术转染绿色荧光蛋白基因作为显色手段,分析在随机及有序纤维支架上DRGn神经突的生长能力以及神经突和电纺纤维的依存关系。结果大鼠原代DRGn能够顺利在PMMA材料上贴壁并生长,在培养第2天,较之平面环境,DRGn未见平均神经突数量及神经突长度的明显区别,电纺纤维的拓扑结构对于DRGn神经突的生长具有明显的接触引导作用,在有序电纺纤维上DRGn能够生成和基质纤维延伸方向一致的神经突;通过GFP表达与背景纤维的合成图,发现在随机或有序电纺纤维上,DRGn神经突均能循纤维向前生长,但相比随机纤维,神经突似乎更倾向于接受有序电纺纤维的引导。结论有序PMMA电纺纳米纤维具有作为神经损伤后大鼠DRGn负载支架的潜力。

英文摘要：

Objective To evaluate the feasibility of electrospun polymethyl methacrylate（ PMMA）nanofibers with different topographic features as scaffolding for rat dorsal root ganglion neurons（ DRGns）.Methods Ordered and disordered electrospun PMMA nanofibers were prepared to culture rat DRGns,with a PMMA film as control. Green fluorescent protein（ GFP） gene as reporter gene was introduced into the DRGns through lentivirus to reveal the growth ability of the DRGns on different substrates,and to analyze the relationship between the neurites and the electrospun nanofibers. Results DRGns could adhere to and grow on the PMMA materials,and the topographic features could exert obvious contact guidance for neurite growth.DRGns cultured on the ordered nanofibers could form ordered neurites as compared with those cultured on the PMMA film and the disordered nanofibers,however,there were no differences in the average number and length of the neurites on the 2nd day. The merged images of GFP fluorescence and the nanofibers showed that the neurites were elongated along the nanofibers,and the ordered nanofibers could provide more support for the DRGns than the disordered nanofibers. Conclusion Ordered electrospun PMMA nanofibers have the potentiality as scaffolding for transplantable DRGns after neural injury.