中文摘要：

为了研究老年大熊猫肠道菌群的构成,本试验对3只圈养老年大熊猫粪便细菌构建16SrDNA克隆文库,采用限制性内切酶HinfⅠ、MspⅠ对其进行限制性片段长度多态性（restriction fragment length polymorphism,RFLP）及测序分析。结果显示,老年大熊猫肠道细菌主要由变形菌门和厚壁菌门组成;变形菌门中又以大肠埃希氏菌属为主,其次为假单胞菌属、志贺氏菌属、气单胞菌属;而厚壁菌门中以链球菌属为主,其次为魏斯氏菌属、梭菌属;此外还发现一定比例的未培养细菌。本试验第一次建立了较丰富的老年大熊猫肠道菌群的克隆文库,为分析比较各年龄层大熊猫的肠道菌群结构的异同提供了参照,也为合理饲喂老年大熊猫,保障老年大熊猫的健康提供重要信息。

英文摘要：

In order to comparatively analyze the differences and similarities of intestinal flora from all ages of giant pandas,we built 16S rDNA clone library of three captive elderly giant pandas＇fe- cal bacteria and made restriction fragment length polymorphism （RFLP） and sequencing analysis by using restriction enzymes Hinf I and Msp I . The results showed that the intestinal flora of elderly giant panda were mainly attributable to two bacteria：Proteobacteria and Firmicutes. Be- tween them,the main in Proteobacteria of intestinal flora of elderly giant panda were Escherichia coli, followed by Pseudornonas sp. , Shigella and Aerornonas;The main in Firmicutes were Strep-tococcus, followed by Weissella ,Clostridium~Of which there were a certain proportion of bacteria were uncultured bacteria. The article firstly established a rich clone library of intestinal flora from elderly giant pandas, which provided a reference to comparatively analyze the similarities and differences in structure of intestinal flora from all ages of giant pandas, as well as provided the im- portant information about reasonable feeding elderly giant pandas and protecting health of elderly giant pandas.