以一年生红地球葡萄幼苗为材料,采用盆栽模拟盐胁迫环境,设置0.1 g/kg0、.2 g/kg和0.3 g/kg等3个盐分梯度,以不加NaCl(0 g/kg)为对照(CK),研究NaCl胁迫对葡萄幼苗光合作用和叶绿素荧光特性的影响。结果表明:随着胁迫时间的延长,各梯度盐处理下葡萄幼苗叶片净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)均降低,最大光化学效率(Fv/Fm)、潜在光化学效率(Fv/F0)、最大量子产额(Yield)、光合电子传递速率(ETR)、光化学猝灭系数(qP)也同时下降,胞间CO2浓度(Ci)先降后升,而非光化学猝灭系数(qN)则逐步上升。盐胁迫导致光合作用的下降是由气孔限制转变为非气孔限制,同时损伤了PSⅡ反应中心,引起其光能原初捕捉能力和光能同化率减弱,增加了通过热辐射消耗的光能比例,最终导致葡萄幼苗光合作用能力的减弱。
A pot experiment on effects of salt stress on photosynthetic physiology and chlor ophyll fluorescence characteristics of 1-year old grape(Red Globe) seedlings was conducted.The seedlings were treated with NaCl concentrations of 0.1,0.2, and 0.3 g/kg,and with fresh water as the control.The results showed that with treatment time increasing,the net photosynthetic rate,transpiration rate,stomatal conductance and the leaf chlorophyll concent ration all decreased.Furthermore,the determination of chlorophyll-fluorescence parameters revealed the PSⅡ maximal photochemical efficiency(Fv/Fm),the PSⅡ potential photochemical efficiency(Fv/F0),the maximum quanta yield(Yield),the photosynthetic electron transport(ETR),and the photochemical quenching coefficient(qP) decreased under the NaCl stress.The intercellular CO2 concentration first decreased and then increased,and non photochemical quenching of fluorescence(qN) increased.Salt stress led to decreasing of photosynthesis of from stomatal limitation to nonstomatal limitation.Furthermore,the damage of PSⅡ brought inactivation of photosynthesis center,decrease of both primary capture capacity and assimilation efficiency of light energy,increase of lightenergy consumption by means of heat and final weakening of photosynthesis capacity of Red Globe seedlings.