中文摘要：

采用苯丙氨酸解氨酶（PAL）为模型酶,制备了以粗孔微球硅胶为内核的苯丙氨酸解氨酶交联酶聚体（NH-PAL-CLEAs）,优化了制备条件。并研究了NH-PAL-CLEAs的部分催化性能。研究结果表明,当硅胶量为117.75mg,酶液添加量为1mL（2U）,戊二醛浓度为0.2%（体积分数）时,所制备的NH-PAL-CLEAs的最大酶活回收率是25%。与传统的PAL-CLEAs相比,NHPAL-CLEAS无需离心或过滤,只用短时间静置就能被回收重复利用。同时,NH-PAL-CLEAs也表现出较好的操作稳定性。这种制备方法不仅克服传统制备交联酶聚体（CLEAs）颗粒小、难回收,离心或过滤回收易结块的问题,而且缩短了生产周期,减少了能源消耗,提高了固定化酶的利用率,是一种非常具有工业应用前景的固定化酶方法。

英文摘要：

In this study,phenylalanine ammonia lyase（PAL）is used as model,and the cross-linked enzyme aggregates of PAL with crude-pored microspherical silica core（NH-PAL-CLEAs）are prepared.The conditions for the preparation of NH-PALCLEAs are optimized.Moreover,some characteristics of the NH-PAL-CLEAs are examined.The optimized conditions for preparing NH-PAL-CLEAs are as follows：117.75 mg silica,0.2%（v/v）glutaraldehyde and 1 mL free PAL solution（2 U）.Under optimized conditions,the maximum activity recovery of NH-PAL-CLEAs reaches 25%.Compared with PAL-CLEAs,the resulting NH-PAL-CLEAs can be easily separated from the reaction mixture by natural precipitation without centrifugation and filtration treatments.Moreover,NH-PAL-CLEAs show superior operational stability.This technique not only overcomes the difficulty to recover CLEAs due to their small particle sizes and soft for many industrial applications,but also reduces production cycle and energy consumption.As a result,this technique shows superior application prospect for enzyme immobilization.