中文摘要：

在室内评价了表达Cry1Ab杀虫蛋白的转基因抗虫玉米Bt11花粉作为食物对玉米螟赤眼蜂Trichogramma ostriniae雌蜂寿命、繁殖力、子代羽化数和性比的影响。结果显示,Bt11玉米花粉中含有115.13±7.29ng/gFW Cry1Ab杀虫蛋白。饲喂水＋Bt玉米花粉和水＋对照玉米花粉的雌蜂寿命、繁殖力和子代羽化数明显高于只提供水的处理,但性比没有显著差异。饲喂10%蜂蜜水的雌蜂寿命、繁殖力和子代羽化数与饲喂10%蜂蜜水＋Bt玉米或10%蜂蜜水＋对照玉米花粉的处理雌蜂相似,没有显著差异。饲喂水＋玉米花粉和只提供水的各处理的寿命、繁殖力和子代羽化数均显著低于蜂蜜水＋玉米花粉和只提供蜂蜜水的各个处理,但性比显著高。饲喂水＋Bt玉米花粉与水＋对照玉米花粉之间以及蜂蜜水＋Bt玉米花粉与蜂蜜水＋对照玉米花粉之间的寿命、繁殖力和子代羽化数没有显著差异。由此得出结论,同只提供水相比,玉米花粉可以提高玉米螟赤眼蜂的寿命和繁殖力。表达Cry1Ab杀虫蛋白的Bt11玉米花粉对玉米螟赤眼蜂的寿命、繁殖力、子代羽化数和性比无不利影响。

英文摘要：

The effect of transgenic Bt corn pollen expressing CrylAb toxin （event Bt 11） as a diet on longevity and fecundity of Trichogramma ostriniae Chen et Pang （Hymenoptera： Trichogrammatidae） were assessed in the laboratory. The results showed that the amount of CrylAb toxin detected in Bt corn pollen was 115.13 ± 7.29 ng/g fresh weight by ELISA method. Females fed on suspension of pollen of transgenic Bt corn or non-Bt corn in water survived for a significantly longer time, parasitized more host eggs, and emerged more offsprings than those fed on water alone, but no significant difference in sex ratio of offspring was observed. The longevity of females fed on 10% honey alone was similar to those fed on suspension of pollen of transgenic Bt corn or non-Bt corn in 10% honey without significant differences in fecundity, number of progeny emerged among the treatments. No significant differences in longevity, number of parasitized eggs, number of progeny emerged and the offspring sex ratio were observed between the females feeding on pollen of Bt corn and non-Bt corn, and this was also shown in experiments with suspension of 10% honey and with water. It was so concluded that corn pollen in water increased the reproduction and survival of T. ostriniae females compared to water alone;corn pollen of event Bt 11 expressing CrylAb toxin had no adverse effect on T. ostriniae.