中文摘要：

目的：建立组织中延胡索乙素的含量测定方法,并研究元胡止痛方白芷有效部位对延胡索有效部位中延胡索乙素在大鼠组织分布特征的影响。方法：灌胃给药,HPLC-FLD法测定大鼠体内延胡索乙素的组织含量,采用甲醇溶剂沉淀蛋白,上清液氮气吹干,流动相复溶,高效液相荧光色谱法测定大鼠心、肝、脾、肺、肾及脑组织中的延胡索乙素。结果：灌胃给予白芷与延胡索有效部位配伍组1h及2h,延胡索乙素在大鼠组织中体内各组织的含量均有不同程度的增加,其增加程度在心、肝、脑部位更为明显。结论：元胡止痛方中白芷可显著提高延胡索中延胡索乙素在靶器官的含量。

英文摘要：

Objective：To develop a method for the determination of tissue distribution of tetrahydropalmatine in rats.The effects of Angelicae dahuricae on the tissue distribution of tetrahydropalmatine of Rhizoma corydalis in complex Yuanhu Zhitong prescription in rats was studied.Methods：The concentration of tetrahydropalmatine in tissues was detected by high-performance liquid chromatography（HPLC） after oral administration.The tissues was pretreated by precipitating protein using methanol,blowing dry clear supernatant liquid,redissolving by mobile phase.Results：After oral administration of active principle of Angelicaedahuricae and total alkaloids of Rhizoma corydalis.The contents of tetrahydropalmatine in rat tissues were increased more or less,with highest concentration found in the heart、liver、brain.Conclusions：In Yuanhu Zhitong formulae,Angelicae dahuricae could increase the concentration of tetrahydropalmatine in target tissues significantly.