中文摘要：

目的：探讨化痰消瘤方含药血清对肺腺癌A549细胞增殖的影响。方法：体外培养肺腺癌A549细胞,分为化痰消瘤方低剂量组、中剂量组、高剂量组,环磷酰胺组,空白组。分别于含药血清中培养24h、48h、72h后,进行观察和检测,独立重复实验3次。采用MTT法检测细胞增殖情况,显微镜下观察细胞形态学变化。结果：各药物干预组吸光度在同一时段内与空白组比较,差异均有统计学意义（P〈0.05）;化痰消瘤方各剂量组吸光度同一时段内与环磷酰胺组比较,差异均有统计学意义（P〈0.05）。化痰消瘤方含药血清对A549细胞增殖的抑制率随药物浓度的增加不断提高。除化痰消瘤方低剂量组外,其余各组随着时间的延长,对A549细胞增殖的抑制率不断提高。显微镜下可见化痰消瘤方各剂量组肺腺癌A549细胞出现胞膜皱缩内陷的细胞凋亡征象。结论：化痰消瘤方含药血清可抑制A549细胞增殖,且具有一定的浓度及时间依赖性,同时可诱导细胞凋亡。

英文摘要：

Objective：To research the effect of Huatan Xiaoliu Prescription containing serum on proliferation of lung carcinoma A549 cells. Methods： Lung adenocarcinoma A549 cells were cultured in vitro and divided into Huatan Xiaoliu Prescription low dose group, middle dose group, high dose group, eyclophosphamide group, blank group. Observed and detected respectively in the drug-containing serum cultivation after 24 h, 48 h, 72 h, independent repeat experiment for 3 times. Cell proliferation was de- tected by MTT method, the morphological changes were observed under the microscope. Results：The drug intervention group ab- sorbance in the same period compared with blank group, the difference had statistical significance （ P 〈 0.05 ） ; Huatan Xiaoliu prescription each dose group absorbance in the same period compared with cyclophosphamide group, the difference had statistical significance （ P 〈 0.05 ）. Huatan Xiaoliu Prescription containing serum inhibition on A549 cell proliferation rate increased with in- creasing drug concentration. Except for the low dose group, the inhibition rate of A549 cell proliferation was increased with the ex- tension of time. Microscopically visible Human Xiaoliu Prescription each dose group of A549 cells in cell membrane shrivel invagi- nation apoptosis signs. Conclusion ： Huatan Xiaoliu Prescription containing serum can inhibit the proliferation of lung adenocarci- noma A549 cells in dose and time-dependent manner,and can induce apoptosis.