中文摘要：

目的比较背部皮下与肾包囊内2种不同移植方式所建立的类风湿关节炎（RA）滑膜侵蚀软骨联合免疫缺陷（SCID）模型的病理学及血清学改变，为采用人滑膜和软骨进行RA炎症及软骨损伤机制和治疗的体内研究提供实验系统。方法15只SCID小鼠随机分为皮下移植组、肾包囊移植组及空白对照组，无菌获取RA患者滑膜及人正常软骨，通过相应的移植方式共同植入各组SCID小鼠体内。分别于术后4、8周取移植组织，进行组织病理学分析；留取血清，用酶联免疫吸附试验（ELISA）检测人类风湿因子（RF）水平。结果皮下移植组成活率及建模成功率较肾包囊组明显增高，两组滑膜中炎细胞浸润、滑膜增殖、软骨侵蚀评分及血清RF-IgM水平差异均无统计学意义（P〉0．05）。结论与肾包囊移植相比，背部皮下移植建立的SCID-HuRAg在炎症及骨侵蚀及血清学改变上差异无统计学意义，且操作简便，成功率高，是进行人RA滑膜对软骨侵蚀机制和治疗体内研究的理想动物模型。

英文摘要：

Objective To compare the pathological and serological difference of rheumatoid arthritis （RA） models in severe combined immnnodeficiency （SCID） mice transplanted with synovial tissues from patients with rheumatoid arthritis （SCID-HuRAg mice） established either by renal capsule or subcutaneous back heterotopic transplantation. Methods RA synovium and normal human cartilage were co-implanted subcutaneously into the backs or under the renal capsule of 15 SCID mice. Engrafted tissues and serum were taken at the 4th and 8th week after transplantation.Histopathology and ELISA were performed to compare their histological and serological differences with RA. Results The morbidity and taken rate were significantly increased in the subcutaneous back of the mice group than the renal capsule group. The degree of cartilage erosion as well as the titers of serum IgM type rheumatoid factor suggested no significant difference between the two groups of SCID-HuRAg model developed by different engraft methods. Conclusion Back subcutaneous transplantation SCID-HuRAg model can be an ideal and stable animal model for studies on the pathogenesis and biotherapy of RA.