中文摘要：

目的：观察艾灸对克罗恩病（CD）大鼠结肠黏膜组织MCP-1、NF-κB蛋白表达的影响。方法：采用国际公认的Morris方法制备CD大鼠模型。将动物随机分为空白对照组、CD模型组和艾条灸组,空白对照组、CD模型组不做治疗,艾条灸组选用特制香烟型纯艾条,在距离＂脾俞＂＂中脘＂穴2 cm高处悬灸,每日治疗1次,每次使用1个穴位,两穴交替使用,每次20 min,连续治疗7 d。应用HE染色光镜下观察结肠病理学变化,免疫组化方法观察大鼠结肠黏膜MCP-1、NF-κB p 65和NF-κB p 50蛋白的表达。结果：与空白对照组比较,CD模型组大鼠可见黏膜、腺体缺损或消失,绒毛破坏,黏膜下层充血水肿,溃疡形成;大鼠结肠黏膜组织MCP-1、NF-κB p 50及NF-κB p 65蛋白表达平均吸光度明显增高（P〈0.01）;与CD模型组比较,艾条灸组大鼠结肠黏膜炎症性反应明显改善,主要表现为黏膜下水肿减轻,有少量炎性细胞浸润,肠腺排列较规则,其大鼠结肠黏膜组织MCP-1、NF-κB p 50及NF-κB p 65蛋白表达平均吸光度显著降低（P〈0.05）。结论：艾灸能够下调CD大鼠结肠黏膜MCP-1、NF-κB蛋白的表达。

英文摘要：

Objective To observe the effect of moxibustion on the expression levels of MCP-1 and NF-κB proteins in the colonic mucosa tissue of rats with Crohn＇s disease（CD）. Methods Morris method, which is internationally recognized, was adopted to establish CD rat models. The rats were randomly divided into blank control group, CD model group and moxa stick moxibustion group. In the moxa stick moxibustion group, the cigarette-like moxa sticks were used for suspended moxibustion at the locations 2 cm from Píshū（脾俞 BL 20） and Zhōngwǎn（中脘 CV 12）. The treatment was conducted once a day, lasted for 20 min at each time, and 7 times were needed; one acupoint was used for each time, and the two acupoints were used alternately. Under light microscope, HE staining was used to observe the pathological changes of colon, and immunohistochemical method was adopted to observe the expression levels of MCP-1, NF-κB p 65 and NF-κB p 50 proteins in colonic mucosa tissue of rats. Results Compared with the blank control group, in CD model group, mucosa and glands defect or disappearance, villus damage, submucosal congestion and edema, and elcosis were found; the average optical density of the expression of MCP-1, NF-κB p 50 and NF-κB p 65 proteins in the colonic mucosa tissue of rats significantly increased（P〈0.01）. Compared with the CD model group, in the moxa stick moxibustion group, the inflammatory response of colonic mucosa of rats improved significantly, mainly manifesting as relieved submucosal edema, a small amount of inflammatory cells infiltration, and regular intestinal glands distribution; the average optical density of the expression of MCP-1, NF-κB p 50 and NF-κB p 65 proteins in the colonic mucosa tissue of rats significantly decreased（P〈0.05）. Conclusions Moxibustion can down-regulate the expression levels of MCP-1 and NF-κB proteins in the colonic mucosa of CD rats.