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中文摘要：
将含有牛病毒性腹泻病毒1型(BVDV1)囊膜糖蛋白E2编码基因的真核表达质粒pEC143免疫BALB/c小鼠,应用淋巴细胞杂交瘤技术,以重组E2蛋白纯化产物为检测抗原,经间接ELISA法筛选和3次有限稀释法克隆,得到1株能稳定分泌抗BVDV1和BVDV2单克隆抗体的杂交瘤细胞株。该3D8株单抗能识别BVDV1标准毒株(NADL、OregonC24V)、BVDV2标准毒株(890、104)、2株BVDV1分离株和3株BVDV2分离株,而不能识别牛轮状病毒、牛疱疹病毒1型,显示出良好的特异性。阳性杂交瘤细胞的上清液、腹水ELISA抗体效价分别为29和10×27,为IgM亚类。其单克隆抗体杂交瘤细胞株的获得,为建立单抗介导的检测BVDV1和BVDV2抗原和抗体的血清学方法和实现标准化诊断奠定基础。
英文摘要：
The glycoprotein E2 from bovine viral diarrhea virus genotype 1 or BVDV2 is an immunodominant antigen inducing the neutralizing antibodies after natural infection or following immunization with live or killed vaccines.In this study,E2 glycoprotein was focused and the BALB/c mice were immunized with the recombinant eukaryotic plasmid pEC143 encoding a secreted form of E2 glycoprotein according to the immune protocol for making monoclonal antibody.Using lymphocyte hybridoma technique,the mouse spleen cells we...
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