中文摘要：

为了提高紫色硫细菌283-1的生物量,对其培养基组分进行优化。采用单因子试验、Plackett-Burman设计法、最陡爬坡试验及中心组合设计响应面分析,研究了乙酸钠、碳酸氢钠、氯化铵、硫化钠、硫酸镁和无机盐6个因子对菌株283-1生物量的影响。结果表明,乙酸钠和硫化钠是影响该菌株生物量的主要因素,并获得了最佳培养基配方：乙酸钠1.45g·L-1,碳酸氢钠0.75g·L-1,氯化铵4.5g·L-1,硫酸镁0.5g·L-1,硫化钠1.05g·L-1,氯化钙0.19g·L-1,氯化钾0.34g·L-1,氯化钠50g·L-1,VB1220μg·L-1,无机盐溶液1mL·L-1。优化后菌株283-1的生物量提高了6倍,结果与理论预测值相近。

英文摘要：

In order to improve the biomass,medium composition of the strain 283-1 of purple sulfur Bacterium was opti mized. Effects of the six factors CH：3COONa, NaHCO3, NH3Cl, Na2S, MgSO, and inorganic salt on the biomass were evaluated by using single-factor test, Plackett-Burman design,the steepest ascent experiment and central compos- ite design combined with response surface analysis in this work. The results showed that sodium acetate and sodium sul- fide were the most significant factors and the optimal media were as following： CH：3COONa 1.45 g·L-1 , NaHCO3 0.75 g·L-1 , NH,C1 4.5 g·L-1 , MgSO4 0.5 g·L-1 , Na.,S 1.05 g·L-1 , CaCl2 g·L-1 , KC1 0.34 g·L-1 , NaCI 50 g·L-1, V1312 20 g·L-1 and inorganic salt 1 mL·L-1. With the optimized media, the biomass improved 6 times compared with the control and the value was in agreetment with the predicted.