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Identification of auxin responsive genes in Arabidopsis by cDNA array
  • ISSN号:1001-0602
  • 期刊名称:《细胞研究:英文版》
  • 时间:0
  • 分类:Q943[生物学—植物学]
  • 作者机构:1. Institute of Genetics, Chinese Academy of Sciences, 100101, Beijing, China ,
  • 相关基金:This work was supported by the National Natural Science Foundation of China (Grant Nos. 30070074, 39889003 and 39525017).
中文摘要:

The plant hormone auxin influences a variety of developmental and physiological processes. But the mechanism of its action is quite unclear. In order to identify and analyze the expression of auxin responsive genes, a cDNA array approach was used to screen for genes with altered expression from Arabidopsis suspension culture after IAA treatment and was identified 50 differentially expressed genes from 13824 cDNA clones. These genes were related to signal transduction, stress responses, senescence, photosynthesis, protein biosynthesis and transportation. The results provide the molecular evidence that auxin influences a variety of physiological processes and pave a way for further investigation of the mechanism of auxin action. Furthermore, we found that the expression of a ClpC (regulation subunit of Clp protease) was repressed by exogenous auxin, but increased in dark-induced senescing leaves. This suggests that ClpC may be a senescence-associated gene and can be regulated by auxin.

英文摘要:

The plant hormone auxin influences a variety of developmental and physiological processes. But the mechanism of its action is quite unclear. In order to identify and analyze the expression of auxin responsive genes, a cDNA array approach was used to screen for genes with altered expression from Arabidopsis suspension culture after IAA treatment and was identified 50 differentially expressed genes from 13824 cDNA clones. These genes were related to signal transduction, stress responses, senescence, photosynthesis, protein biosynthesis and transportation. The results provide the molecular evidence that auxin influences a variety of physiological processes and pave a way for further investigation of the mechanism of auxin action. Furthermore, we found that the expression of a ClpC (regulation subunit of Clp protease) was repressed by exogenous auxin, but increased in dark-induced senescing leaves. This suggests that ClpC may be a senescence-associated gene and can be regulated by auxin.

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期刊信息
  • 《细胞研究:英文版》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院上海生物科学研究院 生物化学与细胞生物学研究所
  • 主编:姚錱
  • 地址:上海市岳阳路319号,31B座,401室
  • 邮编:200031
  • 邮箱:
  • 电话:021-54920958 54920953
  • 国际标准刊号:ISSN:1001-0602
  • 国内统一刊号:ISSN:31-1568
  • 邮发代号:4-645
  • 获奖情况:
  • 2001年被推选入国家出版属的《中文期刊方阵》之一,中国期刊方阵“双效”期刊,2003年评为国家出版署百种重点期刊奖,2003-2004,2005-2006两届国际自然基金重点期刊,2002-2005中科院重点期刊,第三届中国出版政府奖
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  • 俄罗斯文摘杂志,美国化学文摘(网络版),荷兰文摘与引文数据库,美国生物医学检索系统,美国剑桥科学文摘,美国科学引文索引(扩展库),美国生物科学数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊
  • 被引量:990