中文摘要：

为了解上海地区猪场蚊媒携带乙型脑炎病毒（JEV）的流行情况，本研究对2014年上海地区猪场采集的4000只蚊媒样品进行了病毒分离、RT-PCR及间接免疫荧光鉴定。结果表明，分别从三带喙库蚊和中华按蚊中分离到两株JEV，命名为MH-7株和PD．19株。E基因序列分析显示MH．7株属于基因I型，PD-19株属于基因III型。与减毒活疫苗株SA-14．14．2株E基因核苷酸和编码氨基酸比较结果显示，MH-7株的同源性分别为87．4％和97．2％；PD．19株为98．1％和97．2％。将两株分离株分别接种3日龄乳鼠，均可在72h之内致乳鼠死亡，表明它们均具有较强的神经毒力。本研究为上海当地制定JEV防制方案提供了实验依据。

英文摘要：

To investigate the prevalence of Janpanese encephalitis virus （JEV） from mosquitoes in Shanghai pigpens, a total of 4,000 mosquitoes were collected in 2014. Followed by viral isolation, then identified by RT-PCR and indirect immunofluorescence assay. Two isolates of JEV were isolated from Culex tritaeniorhynchus and Anopheles sinensis. Sequence analysis based on the amplified E gene indicated that MH-7 was grouped into genotype I, and PD-19 was classified into genotype III. The homology of the nucleotide and amino acid sequence between MH-7 and vaccine strain SA-14-14-2 was 87.4% and 97.2%; PD-19 and vaccine stain was 98.1% and 97.2%. Moreover, all of the suckling mice died within 72 hrs when inoculated with the isolate. This study provided a basis references for Shanghai local JEV prevention.