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中文摘要：
采用NTT法考察了16种稀土元素在9×10^-8-2×10^-4mol·L^-1浓度范围内对体外培养的人正常肝细胞株7701细胞和宫颈癌Hela细胞的生长的影响。结果表明，稀土对细胞生长的影响存在浓度依赖性；低浓度促进细胞增殖，高浓度抑制细胞生长；不同稀土离子对细胞作用强弱不同，稀土间存在轻重分组效应；不同细胞对稀土的响应不同，表现出稀土对正常细胞和癌细胞作用的某种选择性。Hoeehst 33258标记细胞DNA，用激光共聚焦显微镜和流式细胞仪对稀土处理的肝细胞株7701进行形态学观察和DNA含量分析。结果表明，较高浓度的稀土作用后的肝细胞株7701出现了明显的凋亡特征，稀土表现出的细胞毒性作用，其本质是诱导细胞发生凋亡。
英文摘要：
By using MTr assays, the effects of Ln on the growth and viability of human normal liver line 7701 and human cervical carcinoma Hela cell were investigated at concentration range of 2 ×10^-4 to 9×10^-8 mol·L^-1. The results show a concentrations-dependent effect of Ln. Ln inhibits growth of both cell lines at high concentrations, but stimulates proliferation at lower concentration. It was found that the effect of Ln on the cell growth depends on Ln species and Ln concentrations. These results also suggest different cell response between cancer cells and normal cells. Hoechst 33258 was used to stain the nuclear DNA to analysis cell morphology and DNA content. The results of studies by laser scanning confocal microscopy and flow cytometry indicate obvious apoptotic characteristics. The results show that Ln-inhibit growth of human normal liver cell line 7701 is related to Ln-induced cell apoptosis.
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