中文摘要：

目的：探讨微波辐射对小鼠生精细胞损伤机制及金匮肾气丸治疗机制。方法：将50只雄性BALB/c小鼠随机分为正常组,辐射组,金匮1、2、3组。除正常组外,其余4组均暴露于2 450MHz、10m W/cm2连续微波中,1h/d,18d。金匮1、2、3组每次分别给予0.1、0.2、0.4g/kg体质量金匮肾气丸灌胃,正常组、辐射组分别给予0.1m L 0.9%氯化钠溶液灌胃,1次/d,18d。检测小鼠生精细胞内Ca~（2＋）荧光强度、内质网钙通道蛋白（Ry Rs）、线粒体膜电位（MMP）。结果：与正常组比较,辐射组Ca~（2＋）荧光强度显著升高,而MMP、Ry Rs阳性表达显著降低（P〈0.05）;而金匮1、2、3组与辐射组比较,Ca~（2＋）荧光强度降低,MMP、Ry Rs阳性表达显著升高（P〈0.05）,呈浓度依赖型,其中,金匮3组在Ca~（2＋）荧光强度方面改善优于金匮1、2组（P〈0.05）。结论：微波辐射损伤可使生精细胞内Ca~（2＋）浓度增高,MMP、Ry Rs阳性表达降低,金匮肾气丸可通过调节Ry Rs、MMP修复损伤,降低细胞内Ca~（2＋）浓度而达到治疗效果,且药量增加效果显著。

英文摘要：

Objective：To explore the mechanism of microwave irradiation on the damage of spermatogenic cells in mice and the therapeutic mechanism of Jinkui Shenqi Pills.Methods：Fifty male BALB/c mice were randomly divided into normal group,irradiation group,Jinkui group 1,Jinkui group 2,and Jinkui group 3.In addition to the normal group,the other 4 groups were exposed to 2 450 MHz,10mW/cm~2 continuous microwave one hour per day for 18 days.Mice in Jinkui group 1,Jinkui group 2,and Jinkui group 3 received gavage administration with 0.1,0.2,0.4g/kg Jinkui Shenqi Pill respectively,and mice in normal group and irradiation group received gavage administration with 0.1 mL of 0.9%sodium chloride solution once a day for 18 days.The fluorescence intensity of Ca~（2＋） in spermatogenic cells,ryanodine receptors（RyRs）,and mitochondrial membrane potential（MMP）were detected.Results：Compared with the normal group,the fluorescence in tensity of Ca~（2＋） in irradiation group was significal rising while the number of MMP,RyRs positive cells was significal reduced,and the fluorescence intensity of Ca~（2＋） in Jinkui group 1,Jinkui group 2 and Jinkui group 3 were decreasing,the number of RyRs positive cells,and MMP were significal increasing（P〈0.05）,and there were concentration dependence.Compared with Jinkui group 1 and Jinkui group 2,Jinkui group 3 improved significantly in the improving Ca~（2＋） intensity department（P0.05）.Conclusion：Microwave radiation damage can increase Ca~（2＋） concentration in spermatogenic cell,and Jinkui Shenqi Pills can repair the damage by regulating RyRs and MMP,and the therapeutic effect increases with the increase of the dose.