中文摘要：

基于时间分辨荧光各向异性成像技术,利用激光扫 描共聚焦显微镜耦合时间相关单光子计数系统,对不同浓度甘油及不同浓度蔗糖的罗丹明B 溶液粘度的荧光各向异性进行了测量。实验表明,随着溶液浓度增大,荧光偏振值和粘度都 相应增大,而荧光寿命与溶液微环境的粘度成反比。据此,基于荧光分子各向异性的产生原 理,给出了荧光团在转动过程中旋转弛豫时间与旋转相关时间的定量关系。它表明时间分 辨荧光各向异性检测可用于微环境粘度的检测和源自细胞内粘度变化导致的机体某些疾病或 机能失调的诊断。

英文摘要：

In this paper,a confocal laser scanning microscope coupled wi th a time-correlated single photon counting （TCSPC） module is used to measure the fluorescence anisotropy of the Rhodamine B glycerol and sugar solution with various concentrations.The experimental results show that fluorescence polarization and viscosity increase with the concentration increas ing.Moreover,lifetime is inversely proportional to the viscosity in the solution.Based on the results of our experiment,we present th e numerical relationship between the rotational relaxation time and the rotational correlation time.The basic principle of fluo rescence anisotropy analysis technology and relevant theories are introduced.Time-resolved fluorescence anisotropy imaging c an quantitatively measure the anisotropy of the sample microenvironment.We finally predict the viscosity sensitivity in live cells and indicate its future directions and applications.