利用SRAP标记对陕西省杨凌地区36株苹果树腐烂病菌(Valsa mali)基因多态性进行分析。从150对SRAP引物中筛选得到8对多态性高、稳定性好的引物组合对供试菌株进行扩增,共得到多态性条带56条,占总带数的75.68%,36株菌株的相似系数为0.339 6-0.962 2。其中,菌株167和165ZJG之间相似系数最大,亲缘关系最近。菌株SXYL24与SXYL135之间的相似系数最小。UPGMA聚类分析显示,在相似系数为0.846处36株供试菌株被划分为4个类群,与菌株采集来源及致病力不存在相关性。
DNA polymorphism of 36 Valsa mali strains from Yangling,Shaanxi province was analyzed using 8 highly polymorphic and stable primer pairs screened from 150 pairs of SRAP primers.56 polymorphic bands were amplified,accounting for 75.68% of the total.The similarity coefficient of the 36 V.mali strains was 0.339 6-0.962 2.Strains 167 and 165ZJG had the maximum similarity coefficient and the closest relationship.With a genetic coefficient of 0.846,36 strains could be classified into 4 subgroups based on UPGMA cluster analysis.And the cluster results had no relationship with strains collected origin or pathogenicity.