中文摘要：

异黄酮是一类具有C-6／C-3／C-6骨架的二次代谢产物，具有抗氧化和抗肿瘤活性。异黄酮与黄酮类物质具有相似的苯丙烷生物合成途径。天然的绝大部分异黄酮分布在豆科植物中，目前在大豆中已经发现了超过12个异黄酮（苷）。大豆异黄酮的生物合成主要涉及三个关键的酶查尔酮合酶（CHS）、查尔酮异构酶（CHI）和异黄酮舍酶（IFS）。总结了大豆异黄酮的提取分离方法和生物合成途径，着重综述了CHI、CHS、IFS生物学特征和功能及异黄酮的代谢工程研究。

英文摘要：

Isoflavones were one class of secondary metabolites with C-6/C-3/C-6 skeleton, which possessed anti-oxidant and anti-tumor activities. Isoflavones and flavonoids had similar phenylpropanoid biosynthetic pathway. The majority of natural isoflavones occurred in leguminous plants. At present, more than 12 isoflavones were identified in soybean. The biosynthesis of soybean isoflvones mainly involved three key enzymes, which are CHS （chalcone synthase）, CHI （chalcone isomerase）, and IFS （isoflavone synthase）, summarized The extraction and isolation methods and biosynthesis pathway of soybean isoflavones were summarized, and emphatically reviewed the biological characteristic and functions of CHI, CHS, and IFS as well as isoflovnes metabolic engineering were emphatically reviewed.