中文摘要：

目的研究小鼠新生期卡介苗（BCG）接种对呼吸道合胞病毒（RSV）感染后肺部炎症和免疫状态的影响。方法新生BALB／c小鼠分4组：A组为对照组、B组为BCG接种组、C组为RSV感染组、D组为BCG接种后RSV感染组。RSV感染后1周观察肺泡灌洗液（BALF）中白细胞数及分类，ELISA法检测BALF中IFN-γ、IL-4、IL-10含量，RT-PCR检测肺组织Toll样受体4（TLR4）mRNA表达，HE染色进行肺组织炎症病理评分。结果C、D两组BALF中自细胞总数、淋巴细胞数、中性粒细胞数、IFN．7含量以及肺组织TLR4 mRNA表达量和炎症病理评分显著高于A、B组（P〈0．01或0．05），BALF中IL-4、IL-10含量4组间差异无显著性，C、D两组之间在各项指标上差异无显著性。结论小鼠新生期BCG接种对RSV感染诱发的肺组织炎症和免疫状态无影响。

英文摘要：

Objective To study the impact lung inflammation and immune status of mice of neonatal Bacillus Calmette-Guerin （BCG） vaccination on with respiratory syncytial virus （RSV）infection. Methods Neonatal BALB/ c mice were divided into four groups. Group A was control. Group B was BCG-vaccinated only. Group C was RSV-infected only. Group D was BCG-vaccinated and RSV-infected. One week after RSV infection, bronchoalveolar lavage （BAL） was performed. BALF cells were counted and differentiated. BALF IFN-γ, IL-4 and IL-10 levels were detected by ELISA and lung TLR4 mRNA expression was analyzed by RT-PCR.Lung inflammation was scored after HE staining. Results Compared with group A and B, BALF total white cells, lymphocytes, neutrophils and IFN-γ concentration and lung TLR4 mRNA expression and inflammatory scores were significantly higher （P〈0.01 or 0.05） in group C and D. No differences were observed in BALF IL-4 and IL-10 levels between the four groups. There were no significant differences in all parameters between group C and D. Conclusions Neonatal BCG vaccination had no influence on RSV infection-related Thl-type cytokine response, increased TLR4 mRNA expression and pulmonary inflammatory infiltration.