[目的]本文旨在研究不同锌源及锌水平饲粮中添加微生物植酸酶对固始鸡和AA肉鸡生产性能、肝脏和胫骨锌沉积、粪便锌浓度的影响。[方法]选择固始鸡与AA肉鸡公雏各800只,在2种基础日粮(分别含植酸酶与不含植酸酶)中分别添加由硫酸锌、氧化锌和氨酸锌提供0、30、60与120 mg·kg^-1水平锌组成的处理日粮,共计40组,每组4个重复,每个重复10只鸡;植酸酶的添加量为500 FTU·kg^-1;试验期共6周,分别在2、3、4、5和6周龄末屠宰取样,测定生长性能及肝脏、胫骨和粪便中的锌浓度。[结果]日粮中添加微生物植酸酶可极显著提高固始鸡和AA肉鸡体增质量(P〈0.01),植酸酶×品种互作对体增质量存在显著性影响,饲料中添加植酸酶对提高AA肉鸡体增质量效果极显著好于固始鸡(P〈0.01);固始鸡肝脏、胫骨和粪便中锌浓度极显著高于AA肉鸡,饲料中添加植酸酶极显著提高固始鸡和AA肉鸡肝脏和胫骨锌含量(P〈0.01),提高了锌的沉积率;植酸酶×锌水平对肝脏和胫骨锌沉积存在极显著互作效应,对未添加锌的对照组胫骨锌浓度可提高10%以上,而对120 mg·kg^-1添加水平处理组几乎没有提高;饲料中添加微生物植酸酶可降低肉鸡粪锌浓度20.37%,能有效缓解重金属锌对生态环境的污染;微生物植酸酶添加对有机和无机锌源的作用效果没有显著差异,揭示植酸酶对植酸螯合物的解离作用可能不止一种途径。[结论]不同锌源及锌水平饲粮中添加微生物植酸酶对固始鸡和AA肉鸡锌代谢产生显著影响,影响的大小与肉鸡品种、锌添加水平有关,与锌源没有显著关系。
[Objectives]The aim of this study was to investigate effects of phytase supplementation in zinc sources and levels of diet on growth performance,concentrations of zinc in liver,tibia,and feces of Gushi chickens and AA broilers. [Methods]Eight hundred one-day old male Gushi chicken and eight hundred one-day old male AA broilers were random divided into 20 groups respectively,and each group with 4 repetitions of 10 birds. Two basal diets were provided: one containing microbial phytase,and another didn' t containing microbial phytase. Both of them were added three source of zinc( Zn SO4·H2O,Zn O and Zn AA) and four levels of zinc content( 0,30,60 and 120 mg·kg^-1),respectively. The amount of microbial phytase was 500 FTU·kg^-1. The total experiment period was6 weeks. One bird in each repetition was slaughtered when the birds at 2,3,4,5 and 6 week' s old,and both the production performances and zinc concentration in liver,tibia,feces were measured. [Results]The results indicated that: microbial phytase increased significantly daily weight gain of Gushi chickens and AA broilers( P0.01); phytase×variety interaction showed that phytase raised a higher body weight gain in AA broilers than in Gushi chickens. The concentrations of zinc in liver,tibia,and feces of Gushi chickens were significantly more increased than those in AA broilers( P 0. 01). Phytase improved significantly liver and tibia zinc concentration of two varieties of chickens( P0.01),and phytase×zinc level interaction analysis showed that tibia zinc concentrations were increased by 10% compared with control groups that contained 0 zinc levels,whereas tibia zinc concentrations was not affected bysupplementation of phytase in the diet with 120 mg·kg^-1zinc. 20.37% of the zinc concentration declined when birds were fed with microbial phytase diet. The supplementation of phytase decreased significantly concentrations of zinc in feces,suggesting that the supplementation of phytase can relieve contamination of heavy metal for biog