中文摘要：

瞄准：开发绑在为早胃的癌症的察觉使用的胃的癌症的亲密关系肽。方法：一幅肽屏幕被 biopanning 执行 PhD-12 噬菌体显示图书馆，对肿瘤邻近的正常出现胃的 mucosa 的变清的非特定的文件夹和对刚收获的胃的癌症纸巾的获得的选择绑定。选择的肽的指向肿瘤的绑定被界限噬菌体计数，连接酶的 immunosorbent 试金，竞争抑制，荧光显微镜学和半量的分析用癌症纸巾的不同类型在 immunohistochemistry 上证实。结果：约 92.8% 非特定的噬菌体克隆对正常在二轮 biopanning 以后从原来的噬菌体图书馆被减去 -- 出现胃的 mucosa。在以后第三轮积极屏蔽，肽顺序 AADNAKTKSFPV (AAD ) 出现在 25%(12/48 ) 分析噬菌体。为控制肽，这些价值是 6.8

英文摘要：

AIM：To develop an affinity peptide that binds to gastric cancer used for the detection of early gastric cancer.METHODS：A peptide screen was performed by biopanning the PhD-12 phage display library,clearing non-specific binders against tumor-adjacent normal appearing gastric mucosa and obtaining selective binding against freshly harvested gastric cancer tissues.Tumortargeted binding of selected peptides was confirmed by bound phage counts,enzyme-linked immunosorbent assay,competitive inhibition,fluorescence microscopy and semi-quantitative analysis on immunohistochemistry using different types of cancer tissues.RESULTS：Approximately 92.8% of the non-specific phage clones were subtracted from the original phage library after two rounds of biopanning against normal-appearing gastric mucosa.After the third round of positive screening,the peptide sequence AADNAKTKSFPV（AAD） appeared in 25%（12/48） of the analyzed phages.For the control peptide,these values were 6.8 ± 2.3,5.1 ± 1.7,3.5 ± 2.1,4.6 ± 1.9 and 1.1 ± 0.5,respectively.The values for AAD peptide were statistically signif icant（P 〈 0.01） for gastric cancer as compared with other histological classif ications and control peptide.CONCLUSION：A novel peptide is discovered to have a specific binding activity to gastric cancer,and can be used to distinguish neoplastic from normal gastric mucosa,demonstrating the potential for early cancer detection on endoscopy.