中文摘要：

目的探讨枸杞多糖（LBP）对同型半胱氨酸（Hcy）致THP-1单核细胞源性巨噬细胞脂质沉积及内质网应激（ERS）的影响。方法复制THP-1单核细胞源性巨噬细胞模型,共分为以下6组：NC组不做其他处理,Hcy组选用100μmol/L的Hcy干预,20 LBP组选用终浓度100μmol/L的Hcy＋20μg/m L的LBP干预,50 LBP组选用终浓度100μmol/L的Hcy＋50μg/m L的LBP干预,100 LBP组选用终浓度100μmol/L的Hcy＋100μg/m L的LBP干预,200 LBP组选用终浓度100μmol/L的Hcy＋200μg/m L的LBP干预。显微镜下观察巨噬细胞模型是否复制成功;RT-q PCR分析脂肪酸结合蛋白4（FABP4）mRNA表达改变;免疫荧光检测巨噬细胞内FABP4蛋白含量的变化;ELISA检测细胞内氧化低密度脂蛋白（ox-LDL）及内质网应激标志蛋白（CHOP、GRP78、XBP-1）含量的变化。结果成功复制THP-1单核细胞源性巨噬细胞模型。与NC组比较,Hcy组巨噬细胞内ox-LDL及FABP4的含量及内质网应激标志蛋白表达显著增加（P〈0.05,P〈0.01）。而不同LBP作用浓度组间比较,oxLDL含量和FABP4 mRNA水平,50LBP组、100LBP组、200LBP组与Hcy组比较,差异有统计学意义（P〈0.05,P〈0.01）,100LBP组、200LBP组与50LBP组比较差异有统计学意义（P〈0.05）。200 LBP组与Hcy组、50 LBP组比较,GRP78含量差异统计学意义（P〈0.05,P〈0.01）。100 LBP组、200 LBP组与Hcy组比较,CHOP含量差异有统计学意义（P〈0.01）,200 LBP组与50 LBP组比较,CHOP含量差异有统计学意义（P〈0.05）。50 LBP组、100LBP组、200 LBP组与Hcy组比较,Xbp-1含量差异有统计学意义（P〈0.01）,100 LBP组、200 LBP组与50 LBP组比较,Xbp-1含量差异有统计学意义（P〈0.01）。结论随着LBP浓度的增加,可逐渐减少Hcy导致的THP-1单核细胞源性巨噬细胞内的脂质沉积及内质网应激蛋白含量,从而发挥抗动脉粥样硬化的作用。

英文摘要：

Objective To investigate the effect of lycium barbarum polysaccharide（ LBP） on homocysteine（ Hcy）-induced lipid accumulation and endoplasmic reticulum stress in THP-1 monocyte-derived macrophages.Methods The model of THP-1 monocyte-derived macrophages was replicated.Cultured cells were divided into 6groups：the control group（ NC）,the 100 μmol / L Hcy group（ Hcy）,the 100 μmol / L Hcy ＋ 20,50,100,200 μg / m L LBP groups.The macrophage model was verified under microscope.Fatty acid-binding protein 4（ FABP4） mRNA levels were detected by real-time PCR.FABP4 protein levels in macrophages were analyzed by immunofluorescence.Intracellular oxygenized low density lipoprotein（ ox-LDL） and endoplasmic reticulum stress markers（ CHOP,GRP78,XBP-1）levels were measured by ELISA kits.Results The macrophage model was successfully established.Treatments of 100μmol /L Hcy and LBP at various concentrations showed that levels of ox-LDL,FABP4 and endoplasmic reticulum stress markers were higher in the Hcy group than in the NC group.While different levels of LBP diminished the alterations in an apparent dose-dependent manner.Conclusion At increasing concentrations,LBP attenuates homocysteine-induced lipid accumulation and down-regulates the levels of endoplasmic reticulum stress markers,thus playing a role of anti-atherosclerosis.