中文摘要：

piggyBac（PB）转座子的末端反向重复序列（ITR）和转座酶编码序列（PBase）分别克隆于质粒PBll56、atula—pBac—K10．ITR与包含红色荧光蛋白表达序列（RFP）的质粒pmCherry—N1重组构成供体质粒（Donor）；PBase与真核表达载体pEFrF-PGK重组构成辅助质粒（Helper）．使用由供体质粒与辅助质粒组成的PB二元共转染系统转染HEK293细胞，结果表明该二元共转染系统具有较好的转座活性，并且在分子水平验证了转座的发生以及使用反向PCR（IPCR）定位了其中一个转座插入位点在HEK293基因组DNA上的位置．

英文摘要：

The PB inverted terminal repeats （ITR） and PB transposase （PBase） were cloned from the plasmid PBl156 and atub-pBac-K10. The PB binary cotransfection system consisted of a donor and a helper plasmid. The donor plasmid contained the two ITRs in which PBase was replaced by a red fluorescent protein （RFP） marker. The helper plasmid carried the PBase fragment but lacked the ITRs. The result of cotransfection the donor and helper in HEK293 cells indicated that the PB binary cotransfection system is an efficient transposition system. And inverse PCR （IPCR） was performed to recover sequence adjacent to the PB ITR.