中文摘要：

目的：观察X射线照射对小鼠腹腔巨噬细胞IL-18表达的影响。方法：昆明种小白鼠80只．随机分为10组（每组8只），即假照组、4个单次低剂量X射线照射组（0．05、0．075、0．1和0．2Gy）和5个单次高剂量X射线照射组（0．5、1．0、2．0、4．0及6．0Gy），X线全身照射（WBI）后16h处死小鼠，取腹腔巨噬细胞，用RPMI 1640培养基，在37℃、5％CO2培养箱中培养72h，留取上清液，采用ELISA法检测小鼠腹腔巨噬细胞IL-18的分泌量。结果：0．05Gy X射线WBI后，IL-18的表达有所增高，检测值为（59．53±3．68）ng·L^-1，与假照组（51．29±3．26）ng·L^-1。比较差异无显著性（P〉0．05）；0．075GyX射线WBI后，IL-18的表达量增高至（122．78±8．07）ng·^-1，与假照组比较差异有显著性（P〈0．01）；随着X射线辐射剂量增加至0．1、0．2、0．5和1．0Gy，IL-18的表达量增高至（135．32±5．27）、（149．40±16．54）、（200．42±15．42）及（347．59±17．88）ng·L^-1，1．0Gy照射达到峰值，随后IL-18表达呈下降趋势；2．0、4．0及6．0GyWBI后，IL-18的表达量分别为（229．49±14．68）、（253．79±6．69）及（223．32±20．85）ng·L^-1，均高于假照组（P＜0．01）。结论：高、低剂量X射线照射均能诱导小鼠腹腔巨噬细胞IL-18的表达。

英文摘要：

Objective To observe the effect of X-rays irradiation on IL-18 expression in mouse peritoneal macrophages. Methods Eiyghty Kunming white mice were divided into 10 groups （ eight each group） ： shamirradiation, four groups of low-dose irradiation （0.05, 0.075, 0.1 and 0.2 Gy）, and five groups of high-dose irradiation （0.5, 1.0, 2.0, 4.0 and 6.0 Gy）. The mice were killed 16 h after whole body irradiation （WBI）, with X-rays then the peritoneal macrophages were obtained and cultivated with culture medium of RPMI 1640 in culture box with 37℃, 5% CO2 for 72 h. The supernate was gathered to detect the IL-18 expression level in macrophages by ELISA. Results The IL-18 expresson level in sham-irradiation group was （51.29 ± 3.26 ） ng·L^-1, IL-18 expression was enhanced after 0. 05 Gy WBI, but there was no significant difference （P〉0. 05）. The expression level was increased to （122.78±8.07） ng ·L^-1 after 0. 075 Gy WBI, and there was significant difference compared with sham-irridiation group （P〈0.01）. IL-18 expression was increased gradually with the enhancement of X-rays irradiated doses （0.1, 0.2, 0.5 and 1.0 Gy）, and the values were （135.32±5.27）, （149.40± 16.54）.（200. 42±15. 42） and （347.59 ± 17.88） ng·L^-1, respectively. It peaked after 1.0 Gy irradiation, then it decreased after 2.0, 4.0 and 6.0 Gy irradiation, and the values were （229.49±14.68）, （253. 79 ± 6. 69） and （223.32±20.85） ng·L^-1, respectively, but they were higher than that in sham irradiation group （P〈0.01）. Conclusion Both high-dose and low dose X-rays irradiation can stimulate IL-18 expression in mouse peritoneal macrophages.