中文摘要：

通过RACE技术从花生叶片cDNA中克隆到了UDP-葡萄糖基转移酶的基因,命名为AhUGT83A1-like（GenBank注册号为KF411463）。该基因全长为1 530bp,ORF为1 380bp,编码460个氨基酸。序列比对与进化分析结果表明,该序列有保守的UDPGT结构域,并且与其它植物的UGT蛋白同源性较高。荧光定量PCR结果表明,AhUGT83A1-like基因在高盐和低温处理的花生根和叶片中均上调表达;在干旱处理时,根中表达也受到显著上调,但叶片中表达量则有明显下降。以上结果表明AhUGT83A1-like可能参与了花生对干旱、低温和高盐的抗性调控。ABA处理结果表明,AhUGT83A1-like在花生根中对ABA响应明显,但在叶片中对ABA没有明显响应,表明该基因在花生根中对非生物胁迫的调控可能是以依赖ABA的方式发挥作用的。

英文摘要：

UDP - glucosyltransferase gene was cloned from peanut leaf using RACE technology and was named AhUGT83A1 -like （GenBank accession KF411463）. The full length cDNA of AhUGT83A1 -like was 1 530bp, ORF 1 380bp, encoding 460 amino acids. Sequence alignment and phylogenetic analysis revealed that AhUGT83A1 - like had the conserved UDPGT domain and shared high homology with UGT proteins from other plant species. Expression analysis indicated that AhUGTB3A1 - like was induced in peanut roots and leaves under both cold and salt conditions. The expression of AhUG783A1 - like was increased in drought - treated roots, but decreased in drought - treated leaves. The results suggested AhUGT83A1 - like may be involved in abiotic stress regu- lation of peanut. In addition, AhUGT83A1 -like was induced by ABA in peanut roots, which indicated that AhUGT83A1 - like protein might have participated in abiotic stress regulation in the ABA - dependent pathway in peanut roots.