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杂色鲍野生群体与养殖群体自交和杂交子一代的RAPD分析
  • ISSN号:2095-9869
  • 期刊名称:《渔业科学进展》
  • 时间:0
  • 分类:S968[农业科学—水产养殖;农业科学—水产科学]
  • 作者机构:[1]海南大学海洋学院教育部热带生物资源重点实验室海南省热带水生生物技术重点实验室,海口570228
  • 相关基金:国家自然科学基金项目(30511140294)和海南省自然科学基金项目(80412)共同资助
中文摘要:

杂色鲍野生群体和养殖群体分别自交和杂交产生野生鲍自繁F1代(WW)、养殖鲍自繁子代(CC)、正交子一代(W旱×C♂,简称WC)和反交子一代(C♀×W♂,简称CW)4个群体,采用RAPD分析4个群体遗传特性。就多态位点比例、Nei基因多样性值和Shannon信息指数而言,WW群体比CC群体高;杂交群体WC的值分别为88.76%、0.3246和0.4811,比其余群体都要高,与群体WW最接近;另一杂交子代群体CW除多态位点比例比群体CC高外,其余参数均为最低。群体间的遗传距离分析表明,WW与各群体的遗传距离都较大,其中与CC群体的遗传距离最大,为0.0849,与两个杂交群体间的遗传距离分别为0.0772(CW)和0.0671(WC);CC群体与两个杂交群体间的遗传距离都较小,分别为0.0610(与CW)和0.0597(与WC);杂交子代间的遗传距离为0.0600;杂交群体WC与WW的遗传距离大于与CC的遗传距离,CW与WW的遗传距离也大于与CC间的遗传距离,两个杂交群体与亲本的遗传距离不对等,偏向养殖亲本。

英文摘要:

Genetic differences of the first generation from inbreeding and crosses of wild and cultured abalone, Haliotis diversicolor Reeve were analyzed with the random amplified polymorphic DNA (RAPD) technique. The four populations included WW (wild ♀ × wild ♂ ), CC (cultured ♀ ×cuhured ♂ ), the direct crossing population CW (cultured ♀×wild ♂ ) and the reverse crossing population WC (wild♀ × cultured ♂ ). The ratio of polymorphic loci, Nei's gene diversity and Shannon's Information index of WC were 88.76 %, 0. 324 6 and 0. 481 1, respectively, all of the values being the highest among the four populations. Besides, the three indexes of WW were higher than those of CC. In another crossing population CW, all the indexes were the lowest except that the ratio of polymorphic loci was higher than that of CC. Analy-sis of genetic distance showed that the highest genetic distance (0. 084 9) appeared between WW and CC. The genetic distance was 0. 077 2 between WW and CW, and 0. 067 1 between WW and WC. The genetic distances between CC and both crossing populations were relatively low, viz. 0. 061 0 (between CC and CW) and 0. 059 7 (between CC and WC). The genetic distance between the two crossing populations was 0. 060 0. The results showed that the genetic distances between CC and anyone of the two crossing populations were higher than those between WW and anyone of the two crossing populations, indicating that the genetic distances were not equal between the hybrids and the two parents, but more similar to cultured population

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期刊信息
  • 《渔业科学进展》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国水产科学研究院黄海水产研究所 中国水产学会
  • 主编:唐启升
  • 地址:青岛市南京路106号1号楼210室
  • 邮编:266071
  • 邮箱:yykxjz@ysfri.ac.cn
  • 电话:0532-85833580
  • 国际标准刊号:ISSN:2095-9869
  • 国内统一刊号:ISSN:37-1466/S
  • 邮发代号:24-153
  • 获奖情况:
  • 2002年获全国优秀农业期刊2003年被评为中国科技...
  • 国内外数据库收录:
  • 美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:2849