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孔雀石绿及其代谢物在斑点叉尾（鱼回）体内及养殖环境中的消解规律

ISSN号：1000-6907
期刊名称：《淡水渔业》
时间：0
分类：S948[农业科学—水产养殖;农业科学—水产科学]
作者机构：[1]中国水产科学研究院长江水产研究所农业部淡水鱼类种质监督检验测试中心,武汉430223, [2]中国水产科学研究院,北京100141, [3]华中农业大学水产学院,武汉430070
相关基金：公益性行业（农业）科研专项（201203085）;中国水产科学研究院基本科研业务费专项课题（2012A1102）

作者：杨秋红[1], 刘永涛[1], 艾晓辉[1], 王群[2], 宋怿[2], 索纹纹[1,3], 吕思阳[1,3]

关键词：斑点叉尾鮰(Ietalurus, punetaus), 孔雀石绿(MG), 养殖环境, 消除, 残留, letalurta, punetaus , malachite green （MG） , aquaculture environment , eliminate , residual

中文摘要：

研究了以全池泼洒的投药方式，孔雀石绿（MG）（池塘中MG的理论浓度为1mg／L）及其主要代谢物隐性孔雀石绿（LMG）在斑点又尾（鱼回）（letalurus punetaus）肌肉和皮肤以及养殖水体和底泥中的残留消除规律。采用高效液相色谱串联质谱法（HPLC—MS／MS）分析MG及其代谢物LMG在斑点叉尾（鱼回）体内及环境中的浓度水平。结果显示：肌肉、皮肤中MG于用药后第1天最高浓度分别为：（42．77±5．26）μg/kg和（6．36±0．11）μg/kg，消除半衰期T1／2分别为57．76d、31．51d；皮肤和肌肉中LMG分别在用药后第3天和第1天达到最高（502．27±20．43）μg／kg和（125．26±12．76）μg/kg，消除半衰期T1/2分别为33．01d、38．51d。这表明MG在斑点叉尾（鱼回）体内会迅速转化为LMG，且LMG残留在皮肤中的浓度大于肌肉中的浓度。养殖环境底泥中同时存在MG和LMG，以LMG为主，并且LMG呈现蓄积的趋势，在第360天出现最高浓度（5．92±1．23）μg/kg；水体中MG最高浓度出现在第1天，为（46．44±7．59）μg/kg，随后急剧降至μg/kg左右，水体中几乎不存在LMG。

英文摘要：

The residual and elimination rules of malachite green （ MG ） and its main metabolite leucomalaehite green （LMG） in Ietalurus Punetaus tissue （muscle and skin） and aquaculture environment （water, mud） were studied. We took the method of splashing MG in whole pond, and the concentration of MG was lmg/L theoretically. The concentration of MG and LMG in all samples was determined by liquid chromatography tandem mass spectrometric （HPLC-MS/MS）. The results showed that the concentrations of MG were （ 42. 77 ± 5.26 ） μg/kg and （ 6.36 ± 0. 11 ） txg/kg in muscle and skin on the first day. The elimination half-life of MG in muscle and skin were 57.76 d and 31.51d respectively. The highest concentrations of LMG were （ 502. 27 ± 20. 43 ）μg/kg in skin on the third day, （ 125.26 ±12. 76） μg/kg in muscle on the first day. The elimination half-life of LMG in skin and muscle were 38.51 d and 33.01d respectively. The result showed that MG was converted to its major metabolite rapidly. LMG was easy to accumulate in skin, and residence time was the longest. There were MG and LMG in mud simultaneously, but mainly LMG. LMG showed an accumulation trend in mud. On the 360 d, the concentration of LMG was the highest （5.92 ± 1.23 ） μg/kg. On the first day, the highest concentration of MG in water was （46.44 ±7.39）μg/L, then decreased to about 1 μg/L rapidly. There was almost no LMG in water.

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