中文摘要：

进巨噬细胞的单核白血球的区别是在生理、病理学的条件下面的一个重要过程，位于单核白血球区别的机制下面的 butthe 不是完全清楚的。一些粘附分子被报导了 toplay 在房间区别的一个重要角色。CD44 是调停的一个重要粘附分子 cellcell 和 cellmatrixinteraction，并且参予许多细胞的功能。当 CD44 被报导了显示出在单核白血球和巨噬细胞之间的不同 activatedstates，我们建议 CD44 可以涉及单核白血球区别。在这研究，我们在单核白血球区别探索了 CD44 的角色并且进一步学习了被包含在里面的机制。THP-1 房间(人的 monocytic 白血病房间线) 与 phorbol 12 十四酸盐 13 醋酸盐(PMA ) 被导致在 vitro 建立 monocytedifferentiation 的模型。它被发现那个 CD44 表达式，到玻尿的酸的有约束力的能力显著地被增加，并且 distributionof CD44 在区别期间被变换成簇。聚类的导致 PMA 的 CD44 和由堵住 CD44 的 CD44 高表示 weresuppressed，它导致了 CD14 表示的抑制。ERK1/2 signalwas 的导致 PMA 的 phosphorylation 也由堵住 CD44 压制了。我们的结果建议 CD44 涉及单核白血球区别。mechanismsof 单核白血球区别追随者 CD44 激活可以包括 CD44 高表示并且聚类哪个接着带 ERK1/2 的 tophosphorylation。

英文摘要：

Differentiation of monocytes into macrophages is an import ant process under physiological and pathological conditions, but the underlying mechanism of monocyte differentiation is not completely clear. Some adhesion molecules have been reported to play an important role in cell differentiation. CD44 is an important adhesion molecule that mediates cell cell and cellmatrix interaction, and participates in a wide variety of cellular functions. As CD44 has been reported to show different activated states between monocytes and macrophages, we propose that CD44 may be involved in monocyte differentiation. In this study, we explored the role of CD44 in monocyte differentiation and further studied the mechanisms that were involved in. THP1 cells （human monocyfic leukemia cell line） were induced with phorbol 12myristate 13acetate （PMA） to establish the model of monocyte differentiation in vitro. It was found that CD44 expression and binding capacity to hyaluronic acid were increased significantly, and the distribution of CD44 was con verted into clusters during differentiation. The PMAinduced CD44 clustering and CD44 high expression were suppressed by blocking CD44, which resulted in the inhibition of CD14 expression. PMAinduced phosphorylation of ERK1/2 signal was also suppressed by blocking CD44. Our results suggested that CD44 was involved in monocyte differentiation. The mechanisms of monocyte differentiation following CD44 acti vation may include CD44 high expression and clustering which in turn lead to phosphorylation of ERK1/2.