中文摘要：

目的探讨细胞核因子-κB（NF-κB）和基质金属蛋白酶-9（MMP-9）在1型糖尿病模型INS2AKITA小鼠颈动脉中的表达及意义。方法取INS2AKITA雄性小鼠和同窝出生的野生型C57BL/6雄性小鼠各10只,分别作为1型糖尿病模型组和正常对照组。在两组小鼠第16周龄时麻醉处死,处死前检测空腹血糖、测量体重。处死后取双侧颈动脉标本,分别行HE染色及免疫组化,检测颈动脉内皮NF-κB,MMP-9的表达;采用EMSA检测颈动脉组织NF-κB核蛋白结合活性,Rea-ltime PCR检测颈动脉组织MMP-9mRNA的表达。结果与对照组比较,1型糖尿病模型组血糖明显增高（P〈0.001）,体重明显减轻（P〈0.05）。HE染色结果显示两组均未见内膜明显增生。INS2AKITA小鼠颈动脉内皮NF-κB和MMP-9表达明显高于对照组,NF-κB核蛋白结合活性明显增高（P〈0.01）。与对照组比较,1型糖尿病小鼠颈动脉MMP-9mRNA表达明显增强（P〈0.05）。结论 1型糖尿病模型INS2AKITA小鼠造模后16周颈动脉内皮无增生,但NF-κB、MMP-9表达明显增强,NF-κB核蛋白结合活性及MMP-9mRNA表达明显增高,可能是反映1型糖尿病颈动脉粥样硬化早期改变的指标。

英文摘要：

Objective To examine the expression and significance of nuclear factor-κB（NF-κB） and matrix metalloproteinases-9（MMP-9） in the carotid artery of type 1 diabetes model INS2AKITA rats.Methods Ten INS2AKITA male rats and ten wild-type C57BL/6 male rats in the same litter were selected as Type 1 Diabetes Model group and contrast group,respectively.The two groups were anesthetized and sacrificed at week 16 after inspection of fasting blood glucose and weighing.Hematoxylin and eosin（HE） staining was performed on carotid artery samples taken from the two groups.Immunohistochemistry was performed to examine NF-κB and MMP-9 protein expressions in endothelial cells of the carotid artery.The NF-κB DNA-binding activity of the carotid artery tissues was inspected by electrophoretic mobility shift assay.MMP-9 mRNA in the carotid artery tissue was measured by real-time polymerase chain reaction.Results Compared with the contrast group,blood glucose rose（P〈0.001） and body weight dropped significantly（P〈0.05） in the type I diabetes model group.The HE staining result shows that the two groups have no apparent intimal hyperplasia.The NF-κB and MMP-9 protein expressions in the endothelial cells of the carotid artery of INS2AKITA rats were higher than those of the contrast group,and NF-κB DNA binding activity increased（P〈0.01）.Compared with the contrast group,the expression of MMP-9 mRNA in carotid artery of type 1 diabetes model increased（P〈0.05）.Conclusions Intimal hyperplasia was not found in the endothelium of the carotid artery of type 1 diabetic rats at week 16.However,NF-κB and MMP-9 protein expression increased.The NF-κB DNA binding activity and MMP-9 mRNA remarkably increased in the carotid artery,which may be possible early abnormal signs for type 1 diabetic atherosclerosis in the carotid artery.