为了解小麦近缘种的醇溶蛋白多样性分布规律,应用A—PAGE方法对63份普通小麦厦其近缘种材料进行醇溶蛋白遗传多样性分析。结果表明,电泳出现99奈迁移率不同的谱带,构成63种组合,总体多态性信息指数达到0.984,以ω区最高,γ和β次之,α区最低。不同基因组构成材料多态性信息指数的分区比较发现,近缘种材料与普通小麦在α区相差最大,其中AA、AABB、AAGG基因组在α区的多态性信息指数均比地方小麦和斯卑尔脱小麦高0.164,而与广泛杂交改良的高代品系类似。谱带分布频率分析发现,高频带和中频带主要出现在ω、γ和β三个区,频率低于0.05的低频带主要出现在α区。聚类分析反映的亲缘关系基本和进化一致,但近缘种与普通小麦高代品系有交叉。此外,本文亦时小麦近缘种的醇溶蛋白分布规律及其在品质育种中的应用潜力进行了讨论。
The gliadin composition of 63 accessions of common wheat(Triticum aestivurn L. )and related species was analyzed by A-PAGE in order to disclose their genetic diversity. Total of 99 gliadin bands with different mobilities and 63 gliadin patterns were observed. The polymorphie information content (PIC) in total was 0. 984, while they are decreased in the order of ω-gliadins, γ and β-gliadins to α-gliadins. The largest difference of different genomes in PIC is that genome AA, AABB and AAGG are higher than landraces and Triticum spelt L. , which have pure genomes AABBDD, and similar to the cultivated lines that was improved with wide cross. Band with highest or higher frequence tending to be ω-gliadins, γ-gliadins and β-gliadins. While band with low frequence or rarely appeared are easy to be found in α-gliadins. The cluster reflected the genetic distance although some relatives closely related and new cultivated lines of common wheat can not be distinguished by gliadins. The distribution of different giliadin in related species of different genomes and it is potential use in improvement of common wheat was discussed.