二种抗菌剂物质由杆菌生产了敏锐紧张 B11 被沸腾, DEAE 52 阴离子交换层析和铝净化氧化物吸附层析。这些净化的物质在硅石胶化薄层色谱(TLC ) 上显示出仅仅一个点。抗菌剂试金证明抗菌剂物质 A 和 B 禁止了象镰刀霉 oxysporum Schl f.sp 那样的植物病原体的生长。niveum, Rhizoctonia 塘鹅 i, Ralstonia solanacearum 和 Xanthomonas oryzae pv。oryzae。另外,抗菌剂物质 B 能也禁止 Magnaporthe grisea 的生长。这二种抗菌剂物质象 121 ° C 一样高对朊酶和温度抵抗。
Two antimicrobial substances produced by Bacillus subtilis strain B 11 were purified by boiling, DEAE 52 anion exchange chromatography and aluminum oxide adsorption chromatography, These purified substances showed only one spot on silica gel thin layer chromatography (TLC). Antimicrobial assays showed that antimicrobial substances A and B inhibited the growth of plant pathogens such as Fusarium oxysporum Schl f.sp. niveum, Rhizoctonia solani, Ralstonia solanacearum and Xanthomonas oryzae pv. oryzae. In addition, antimicrobial substance B could also inhibit the growth of Magnaporthe grisea. These two antimicrobial substances were resistant to proteolytic enzymes and temperature as high as 121℃.