中文摘要：

根据在GenBank中登录的烟草、番茄等的醇酰基转移酶基因的保守序列设计引物，利用RT—PCR和RACE（Rapid Ampllfieation of cDNA Ends，cDNA末端快速扩增）技术从甜瓜白交系M01—3花后25d的果实总RNA中扩增到甜瓜醇酰基转移酶基因全长cDNA。该基因全长为1429bp，开放读码框为1383bp，编码461个氨基酸，GenBank中登录号为EU431334。利用荧光定量PCR方法进行了该基因在果实发育过程中的表达特性分析，结果表明该基因在花后15d果实中的表达量最低，成熟果实中表达量最高。

英文摘要：

PCR primers were designed based on the conserved domain of some alcohol acyl - transferase genes in C, enBank. The full - length eDNA clone was amplified from the total RNA isolated from 25 - day muskmelon fruit after pollination using RT - PCR and RACE. The clone contained 1 429 nucleotides with an open reading frame of I 383 nucleofides. Quantitative real - time RT - PCR analysis indicated that CmAATI mRNA accumulation showed the minimum level on the 15th day after pollination and reached the highest level in mature fruit.