本文以苏丹红Ⅰ为模板分子,以甲基丙烯酸(MAA)、4-乙烯基吡啶(4-VPy)为功能单体,制备了两种模板聚合物(MAA—MIP和4-VPy-MIP)。对4-VPy—MIP进行了Scatchard方程分析。分别以这两种模板聚合物为固相萃取材料来填充固相萃取柱,从掺有苏丹红Ⅰ的红椒粉中萃取苏丹红Ⅰ。本文优化了固相萃取条件。高效液相色谱检测表明,在合适的萃取条件下,采用填充4-VPy—MIP的固相萃取柱可以有效地从红椒粉中分离富集苏丹红Ⅰ。
In this paper,two kinds of molecularly imprinted polymers (MIPs) were prepared with different fimctional monomers as methacrylic acid(MAA) and 4-vinylpyridine(4-VPy). The polymeric material was characterized by scanning electronic microscope and Scatchard analysis. The polymeric particles were packed into 6cm glass columns to be invested to absorb selectively Sudan Ⅰ from red peppers. Under the extraction conditions, the high performance liquid chromatography (HPLC) indicated the MISPE packed with 4-Vpy-MIP possessed excellent selectivity toward the template comparing the MAA-MIP.