中文摘要：

目的制备大青叶、板蓝根药物性口罩,用药物性口罩过滤H1N1流感病毒液,检测滤液中的流感病毒活性,以评价药物性口罩抗流感病毒的效果。方法将血凝效价为2^-12的H1N1流感病毒液通过药物性口罩,收集滤液,采用血凝试验测定H1N1流感病毒的血凝效价;用滤液接种鸡胚,48h后取尿囊液做血凝试验,测定血凝效价及EID50,判断虑液中流感病毒的活性。结果血凝效价为2^-12的H1N1流感病毒液通过药物性口罩后,其血凝效价降为2^-3,与滤过前的流感病毒液血凝效价比较差异有统计学意义（P〈0.01）;滤液接种鸡胚后的血凝效价降为2^-6,与未过滤流感病毒液接种鸡胚后的血凝效价比较差异有统计学意义（P〈0.01）。未浸药口罩滤液对照EID50为10^-4.95/0.1ml,浸药口罩病毒滤液EID50为10^-2.10/0.1ml,二者比较,差异有统计学意义（P〈0.01）。结论大青叶、板蓝根药物口罩,可使93%以上的滤过流感病毒失去感染性,该药物性口罩可用于预防流感病毒的感染。

英文摘要：

Objectives To prepare a mask from extracts of Isatis leaf and root, filter a solution of influenza virus through it, and then detect viral activity in the filtrate in order to evaluate the mask＇s effectiveness against the influenza virus. Methods A solution of H1N1 influenza virus with a hemagglutination titer of 2-12 was filtered through the drug mask. After the filtrate was collected, the hemagglutination titer of the viral solution was determined by performing a hemagglutination test. Then the filtrate was inoculated into chick embryos. The hemagglutination test was performed with allantoic fluid collected after 48 h. Viral activity in the filtrate was analyzed via the hemagglutination titer and EID50. Results Compared to the unfiltered solution of influenza virus with a hemagglutination titer of 2^-12, the hemagglutination titer of the filtrate was 2^-3 （P〈0.01）. The hemagglutination titer of allantoic liquid was 2^-6 after inoculation with filtrate （P〈0.01）. Compared to the unfiltered H1N1 solution with an EID50 of 10^-4.95/0.1ml, the filtrate had an EID50 of 10^-2.10/0.1ml （P〈0.01）. Conclusion The influenza virus lost more than 93% of its infectivity after it was filtered through a mask made from extracts of Isatis leaf and root. This suggests that the drug mask could be used to prevent infection with the influenza virus.