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Cloning the Dmrtl and DmrtA2 genes of ayu (Plecoglossus altivelis) and mapping their expression in adult, larval, and embryonic stages
  • ISSN号:2095-8137
  • 期刊名称:《动物学研究》
  • 时间:0
  • 分类:S511[农业科学—作物学] S965.226[农业科学—水产养殖;农业科学—水产科学]
  • 作者机构:[1]Ningbo University, Ningbo 315211, China
  • 相关基金:Special Preliminary Study of 973(2008CB117015);Changjiang Scholars and Innovative Research Team Projects(IRT0734);Priority Themes of Major Science and Technology in Zhejiang Province(2009C12077);the K.C.Wong Magna Fund of Ningbo University
中文摘要:

The Dmrt family of genes are involved in sex differentiation in different species of invertebrates,and some vertebrates including human.In this study,we cloned the full-length cDNA of ayu(Plecoglossus altivelis)Dmrt1 and DmrtA2.Sequence and phylogenetic tree analyses showed ayu Dmrt1 showed highest similarity to that of Oncorhynchus mykiss while ayu DmrtA2 is most similar to that of Oryzias latipes.Fluorescence-based quantitative reverse transcription PCR(qRT-PCR)revealed the Dmrt1 was predominantly expressed in the testis.At the larval stages,Dmrt1 mRNA expression level was highest during 52–64 days post hatching(dph)and at the gastrula stage during embryonic development.DmrtA2,meanwhile,was specifically expressed in the ovary and was highly expressed in the female brain tissue,but not male brain tissue.During the larval stages,DmrtA2 expression remained high before day 34,and then fluctuated while generally decreasing.During embryonic development,DmrtA2 expression increased gradually and peaked at the hatching stage.Our data suggest that ayu Dmrt1 might participate in the differentiation and maintenance of testis while DmrtA2 may play a role in ovary-differentiation and mature-ovary maintenance.DmrtA2 might also participate in brain development.

英文摘要:

The Dmrt family of genes are involved in sex differentiation in different species of invertebrates, and some vertebrates including human. In this study, we cloned the full-length eDNA of ayu (Plecoglossus altivelis) Draft1 and DmrtA2. Sequence and phylogenetic tree analyses showed ayu Dmrtl showed highest similarity to that of Oncorhynchus mykiss while ayu DmrtA2 is most similar to that of Oryzias latipes. Fluorescence-based quantitative reverse transcription PCR (qRT-PCR) revealed the Draft1 was predominantly expressed in the testis. At the larval stages, Dmrtl mRNA expression level was highest during 52-64 days post hatching (dph) and at the gastrula stage during embryonic development. DmrtA2, meanwhile, was specifically expressed in the ovary and was highly expressed in the female brain tissue, but not male brain tissue. During the larval stages, DmrtA2 expression remained high before day 34, and then fluctuated while generally decreasing. During embryonic development, DmrtA2 expression increased gradually and peaked at the hatching stage. Our data suggest that ayu Dmrtl might participate in the differentiation and maintenance of testis while DmrtA2 may play a role in ovary-differentiation and mature-ovary maintenance. DmrtA2 might also participate in brain development.

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期刊信息
  • 《动物学研究》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院昆明动物研究所
  • 主编:
  • 地址:昆明市教场东路32号中国科学院昆明动物研究所
  • 邮编:650223
  • 邮箱:zoores@mail.kiz.ac.cn
  • 电话:0871-5199026 5113532
  • 国际标准刊号:ISSN:2095-8137
  • 国内统一刊号:ISSN:53-1229/Q
  • 邮发代号:64-20
  • 获奖情况:
  • 2009年第三届云南省优秀期刊奖,1996年荣获云南省优秀科技期刊二等奖,2000荣获中国科学院优秀期刊三等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,美国生物医学检索系统,美国剑桥科学文摘,美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),瑞典开放获取期刊指南,中国北大核心期刊(2000版)
  • 被引量:10857