中文摘要：

目的：探讨葛根素对自发性高血压大鼠（SHR）脑微血管内皮细胞增殖的影响及其信号转导机制。方法：体外培养SHR及正常血压（WKY）大鼠脑微血管内皮细胞,随机分为（1）WKY对照组：以20%丙二醇孵育24h;（2）SHR对照组：同上处理;（3）葛根素组：SHR内皮细胞以不同浓度（25、50、100ng/L）葛根素分别孵育24h;（4）胎牛血清（FBS）组：SHR内皮细胞以10%FBS孵育24h;（5）PD98059＋葛根素组：SHR内皮细胞以丝裂素活化蛋白激酶（MAPKs）抑制剂PD98059（50μmol/L）预孵育10min,再以100ng/L葛根素孵育24h;（6）蛋白磷酸酶激动剂（BDM）＋葛根素组：培养的SHR内皮细胞以蛋白磷酸酶激动剂2,3-丁二酮肟（20mmol/L）预孵育10min,再以100ng/L葛根素孵育24h。采用[^3 H]-胸腺嘧啶核糖核苷酸（[^3 H]-TdR）掺入法测定各组细胞增殖,采用免疫印迹法检测各组细胞p42/44MAPKs磷酸化水平。结果：50ng/L和100ng/L葛根素组SHR大鼠微血管内皮细胞[^3H]-TdR掺入值较SHR对照组分别高74.1%和96.5%（均P〈0.05）,与FBS组比较差异无统计学意义（P〉0.05）。PD98059和BDM＋葛根素组[^3 H]-TdR掺入值分别较100ng/L葛根素组低44.7%和47.5%（均P〈0.05）,与SHR对照组水平无统计学差异（P〉0.05）。25、50和100ng/L葛根素组p42MAPK磷酸化水平较SHR对照组分别高25.0%、66.7%和75.0%（均P〈0.05）,p44MAPK磷酸化水平较SHR对照组分别高17.8%、60.2%和62.7%（均P〈0.05）。PD98059和BDM＋葛根素组p42MAPK和p44MAPK磷酸化水平均较100ng/L葛根素组明显下调（P〈0.05）,而与SHR对照组差异无统计学意义（P〉0.05）。结论：葛根素能诱导SHR脑微血管内皮细胞增殖,其细胞内信号转导可能与p42/44MAPKs磷酸化途径有关。

英文摘要：

Objective：To investigate the effects of puerarin on proliferation of cerebral microvascular endothelial cells（MECs）from spontaneously hypertensive rats（SHR）and its signaling pathway.Method：Cultured cerebral MECs from SHR and WKY rats were randomly divided into experimental groups as follows：（1）WKY control：MECs from WKY rats were incubated with vehicle（20% propanediol）for 24h.（2）SHR control：incubated MECs from SHR with 20% propanediol for 24h.（3）puerarin group：incubated with puerarin（25,50,100ng/L）for 24h.（4）FBS group：incubated MECs from SHR with 10% FBS for 24h.（5）PD98059＋puerarin group：pretreated MECs from SHR with PD98059for 10min before puerarin（100ng/L）treatment.（6）BDM＋puerarin group：pretreated with 2,3-butanedione monoxide（BDM）10min before puerarin（100ng/L）treatment.Cell proliferation was detectedby[^3 H]-TdR incorporation,and phosphorylation of p42/44mitogen-actived protein kinases（p42/44MAPKs）were detected by western blot analysis.Results：[^3 H]-TdR incorporation in MECs from puerarin（50and 100ng/L）groups showed 74.1%and 96.5%increase,respectively,compared with SHR control（P〈0.05）and similar to that from FBS group（P〉0.05）.[^3 H]-TdR incorporation in MECs from PD98059and BDM groups decreased by44.7%and 47.5%,respectively,compared with that from puerarin（100ng/L）group（P〈0.05）,and there were no statistic significance compared with SHR control（P〉0.05）.The phosphorylation of p42MAPK in MECs from puerarin（25,50,and 100ng/L）groups increased by 25.0%,66.7%,and 75.0%,respectively,compared with SHR control（P〈0.05）.The phosphorylation of p44 MAPK in MECs from puerarin（25,50,and 100ng/L）groups increased by 17.8%,60.2%,and 62.7%,respectively,compared with SHR control（P〈0.05）.PD98059and BDM down-regulated puerarin-induced phosporylation of p42/p44MAPKs,compared with puerarin（100ng/L）group（P〈0.05）,which was similar to that from SHR control（P〉0.05）.Conclusion：Puerarin induce