中文摘要：

目的建立珠子参蛋白质和氨基酸的含量测定方法,并比较不同产地珠子参中蛋白质和氨基酸含量的差异。方法实验采用紫外可见分光光度计和全自动氨基酸分析仪,对珠子参中的蛋白质、氨基酸成分进行分析测定。结果陕西、四川、云南三组珠子参总蛋白质含量之间有一定的差异。其中,珠子参总蛋白质的含量顺序为：云南组〉陕西组〉四川组;氨基酸的含量顺序为：四川组〉陕西组〉云南组。氨基酸中甘氨酸项,陕西、四川与云南的珠子参有显著性差异,陕西与四川没有显著性差异。其余氨基酸指标均没有显著性差异。结论将三大产区珠子参的蛋白质、氨基酸含量比较后发现,云南与四川产珠子参蛋白质含量差异明显;陕西、四川与云南产区珠子参氨基酸含量差异明显。本实验建立的珠子参中蛋白质和氨基酸含量的测定方法简便、稳定、可行。

英文摘要：

Objecttive To establish the method testing the content of protein and amino acid in Zhuzishen（ Rhizoma Panacis Majoris） and compare the content difference of it from different regions. Methods The protein and amino acid in Zhuzishen was analyzed and determined by UV visible spectrophotometer and automatic amino acid analyzer. Results The findings showed that the total content of protein in Shaanxi,Sichuan and Yunnan groups is different to certain extent. The order of the total content of protein： Yunnan group Shaanxi group Sichuan group; the of total amino acid： Sichuan group Shaanxi group Yunnan group. As for glycine in amino acids in Zhuzishen,the three groups were significantly different,while the comparison of Shaanxi and Sichuan groups did not have significant difference. All other indexes in amino acids had no marked difference. Conclusion After comparison,the proteins in Yunnan and Sichuan Zhuzishen are significantly different,while the amino acids in Shaanxi,Sichuanand Yunnan Zhuzishen are quite diverse. The determining method setup in the experiment is simple,stable and feasible.