中文摘要：

目的：检测脂多糖作用于树突状细胞前后CD44、CD24基因的表达情况。方法：从外周血分离单个核细胞,加入rhGM-CSF及rhIL-4,培养的第6天,实验组加入脂多糖刺激24 h而对照组不加,于刺激后不同时间点分别提取细胞总RNA,行real time PCR检测。结果：单个核细胞经GM-CSF及IL-4诱导6d后分化成为树突状细胞,表达较低水平的CD44及CD24。脂多糖刺激后树突状细胞的CD44及CD24表达均较对照组增高,CD44增高更明显。结论：树突状细胞受脂多糖刺激后CD44及CD24表达显著增高,这有利于DC-T细胞簇的形成及抗原的递呈,从而导致DC免疫功能的增强。

英文摘要：

Objective：To detect the genes expression for CD44 and CD24 on dendritic cells（DC） before and after stimulation by lipopolysaccharide（LPS）.Methods：Human monocytes were isolated from heparinized whole blood and then induced into DC by adding rhGM-CSF and rhIL-4 for 6d.After that,DCs were induced with or without LPS for 24h and total cellular RNA were extracted for real time PCR analysis.Results：Expressions of CD44 and CD24 were lower in monocytes-derived DC induced by rhGM-CSF and rhIL-4 for 6d.After being stimulated by LPS for 24h,the expression of CD44 and CD24 increased significantly（P0.05）.Conclusion：The expression of CD44 and CD24 on DC increased after being stimulated by LPS.This is related to functional maturation of DC.