中文摘要：

目的：探讨年龄和惊厥持续时间对惊厥持续状态（SC）后海马细胞色素C（cytC）释放的影响．方法：健康成年（ARs）和生后20d幼龄Wistar鼠（IRs）各80只，经腹腔注射氯化锂-匹罗卡品分别诱发持续惊厥发作30min的SC（30minsc）和3hSC，在SC后3h至7d的6个不同时点上处死动物，采用流式细胞仪检测海马细胞cytC含量．比较两组间的动态变化．结果：①30min SC后3h，两组大鼠海马细胞cytC含量均升高，6h达到高峰，12h后开始回落．两组cytC含量峰值分别为32．47±6．28，33．23±8．66，显著高于对照组（P〈0．01）．②30min SC后7d时，IRs组cytC含量已降至正常，而ARs组仍高于正常对照组（P〈0．05）．在SC后1，3，7d，IRs组海马细胞cytC含量均显著低于ARs组．③3hSC后3．6h，两组海马细胞cytC含量均显著高于30minSC后的相同观察时点（P〈0．05）．经偏相关参数分析，在排除年龄的影响后，不同惊厥持续时间与海马细胞cytC含量呈显著正相关（r=0．66，P〈0．05）．结论：①严重惊厥发作可导致海马细胞cytC的释放．②年龄和惊厥持续时间均是影响惊厥后海马细胞cytC释放的重要因素．③惊厥持续时间越长，海马细胞cytC释放越明显．④与成年鼠不同，幼年鼠海马细胞cytC释放启动后不久即迅速回落，提示幼年脑内可能存在一个主动抑制海马细胞cytC释放的保护性反应．

英文摘要：

AIM： To explore the influences of age and duration of status convulsivus （ SC ） on intracellular cytochrome C （ cytC ） release in hippocampus. METHODS ： Convulsive seizures for 30 min or 3 h （30 min SC or 3 h SC） were induced in 80 infant （20 d after birth） and 80 adult Wistar rats （ IRs ＆ ARs respectively） with lithium-pilocarpine ip. The rats were sacrificed at 6 different time points from the 3 h to 7 d after SC termination. Dynamic change of cytC level in hippocampal cells was investigated by flow cytometry. RESULTS ： ① The level of cytC in hippocampal cells increased in the early stage after 30 min SC, and started to decrease 12 h after SC in both IRs and ARs. The peak level was reached 6 h after SC [ （ 32.47 ±6.28 ） in IRs and （ 33.23 ± 8.66） in ARs）]. Both of them were significantly higher than those of control group （ P 〈 0.01 ）. ② At the 7th day after 30 min SC, the level of hippocampal intracellular cytC in IRs had decreased to the level of normal controls, while the level in ARs was still higher than that of normal controls （ P 〈 0.05 ）. At the 1st, the 3rd, and the 7th day after SC, the levels of hippocampal intracellular cytC in IRs were obviously lower than those in ARs. ③ Compared with the same time point after 30 min SC, the levels of hippocampal intracellular cytC after 3 h SC were much higher in different age groups （ P 〈 0.05 ）. Except the effect of the agerelated difference, there was a positive correlation between the duration of SC and hippocampal intracellular cytC content in partial correlation analysis （ r = 0.66, P 〈 0.05 ）. CONCLUSION ： ① Severe seizure could induce intracellular cytC release in hippocampus. ②Age and duration of SC were important factors influencing intracellular cytC release. ③ The longer the duration of SC was, the higher cytC level was. ④ Compared with ARs,intracellular release of cytC decreased sooner in IRs after SC, which indicated that there could be an internal protective response ag