中文摘要：

目的利用可植人体内的经过修饰的功能化聚氨酯构建两种血管内植入装置，分别植入猪肾下腹主动脉和羊肺中进行基因转染实验，研究其作为基因运载平台的可行性。方法将涂覆胶原的聚氨酯膜通过抗体免疫偶联编码绿色荧光蛋白的腺病毒（AdGFP）制备成聚氨酯坡原膜埋植钮装置并植入猪肾下腹主动脉中，7d后取出埋植钮及其周围组织进行切片、荧光显微镜检测及PCR分析；将抗腺病毒抗体直接通过共价连接在带有硫醇化烷基的功能化聚氨酯膜上实现与AdGFP的免疫偶联，制备成聚氨酯人工瓣膜并植入羊肺中，7d后取出肺部小叶及其周围组织进行荧光显微镜检测及PCR分析。结果在猪体内实验中，植入体表面的新生内膜细胞基因转染率为（14．2±2．5）％；在羊体内实验中，聚氨酯瓣膜小叶上的细胞有（25．1±5．7）％被转染。PCR结果显示，在血液或末端组织中都未检测到GFPDNA。结论这两种血管内聚氨酯基因载运系统能够作为靶向高效载运腺病毒的载体，用于心血管疾病的基因治疗。

英文摘要：

Objective To explore the feasibility of utilizing two implantable devices made from modified polyurethane films with antibody tethered replication-defective adenoviruses encoding for green fluorescent protein （AdGFP） as gene delivery platforms. Methods Intra-aortic button implants of collagen-coated polyurethane films with antibody tethered AdGFP were sutured into the infrarenal aorta of adult pigs and pulmonary valve leaflet in juvenile sheep was replaced by polyurethane pulmonary valve cusp replacement with antibodytethered AdGFP. After seven days, the buttons, prosthetic leaflets, and their surrounding tissues were explanted and evaluated for biocompatibility and AdGFP-mediated gene transfer by fluorescent microscopy and PCR analysis. Results In vivo analysis of gene transfer from collagen-coated polyurethane films in pig infrarenal aorta implants, one week explants of the collagen-coated polyurethane films demonstrated （ 14. 2 ± 2. 5 ） % of neointireal cells on the surface of the implant. In sheep pulmonary valve leaflet replacement studies, polyurethane films with antibody tethered AdGFP vector demonstrated （25. 1 ± 5.7 ） % of cells attached to polyurethane valve leaflets were transduced in one week. PCR analyses showed that GFP DNA was not detectable in blood or distal tissues. Conclusion Site-specific intravascular delivery of adenoviral vectors for gene therapy can be achieved with these two kinds of polyurethane implants utilizing the antivector antibody tethering mechanism.