以4—5片叶的组培香蕉苗和粉蕉苗为材料,采用伤根浸菌液的接种法对分离自番禺、中山、肇庆、珠海、顺德等地的香蕉枯萎病菌株进行了致病性测定,同时观察了各菌株在Komada改良培养基上菌落形态特征.结果表明在广东的确存在香蕉枯萎病菌1号和4号2个小种,认为致病性测定结合Komada改良培养基上菌落形态特征是鉴定香蕉枯萎病菌生理小种的简单、快速而又可靠的方法.
Banana Fusarium wilt, a kind of soilborne vascular disease infected by Fusarium oxysporum f.sp. cubense (E. F. Sm. ) Snyd. & Hans. is threatening the banana and Fenjiao production in Guangdong Province, China. Based on the pathogenicity test on 4-5-leaf tissue-cultured banana plantlets and colony morphology on modified Komada medium, the isolates from the regions of Panyu, Zhongshan, Zhaoqing, Zhuhai and Shunde were identified as F. oxysporum f. sp. cubense race 1 and race 4 respectively. It was proved that the pathogenicity test combined with colony morphology on modified Komada medium was a simple, fast and reliable method for identifying races of Fusarium oxysporum f. sp. cubense.