本实验对筛选自我国特色酿酒葡萄产区的东方伊萨酵母YF-12、发酵毕赤酵母YF-19和季也蒙有孢汉逊酵母YF-28这3株非酿酒酵母的生长特性和发酵特性进行了研究。结果表明:东方伊萨酵母YF-12和发酵毕赤酵母YF-19具有较好的生长特性;在发酵环境耐受性方面,3株非酿酒酵母对高质量浓度葡萄糖和SO_2都有较好的耐受性,在酒精耐受性上季也蒙有孢汉逊酵母YF-28表现较差;对3株非酿酒酵母进行纯种发酵实验后测定挥发性物质发现,东方伊萨酵母YF-12和季也蒙有孢汉逊酵母YF-28产生的挥发性物质较多,而发酵毕赤酵母YF-19产生挥发性物质种类很少。上述结果表明,东方伊萨酵母YF-12显示出较好的生长和发酵特性,适合做进一步产业化开发。
Non-Saccharomyces wine yeasts,widely present on grapes and winemaking equipment,have important effects on wine composition,aroma and flavor.To develop non-Saccharomyces yeasts for industrial winemaking,the growth and fermentation characteristics of three indigenous non-Saccharomyces strains,Issatchenkia orientalis YF-12,Pichia fermentans YF-19 and Hanseniaspora guilliermondii YF-28,were evaluated.The results showed that /.orientalis YF-12 and P.fermentans YF-19 grew better.All of the three strains revealed a certain tolerance to high concentrations of glucose and SO_2.H.guilliermondii YF-28 had poorer tolerance to ethanol.The volatile compound analysis was performed after single species fermentation.The results showed that /.orientalis YF-12 and H.guilliermondii YF-28 produced more volatile compounds than P.fermentans YF-19.Our present study shows the potential of /.orientalis YF-12 as a promising candidate for winemaking.