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中文摘要：
目的：探讨人类吲哚胺2,3-双加氧酶（IDO）对细胞表面HLA-I类分子的影响。方法：用脂质体法将含IDO基因的重组质粒pEGFP-IDO转染293T细胞,用Western blot检测IDOGFP在转染细胞中的表达,荧光抗体染色结合流式细胞术检测细胞表面的HLA-I类分子的表达。结果：Western blot证实转染重组质粒的细胞表达IDO。转染空载体的细胞与转染重组质粒的细胞HLA-A,B,C-PE染色的平均荧光强度分别为299.46±62.65及42.51±8.19,两者相比有显著性差异（P〈0.01）;转染重组质粒的细胞,未加色氨酸组与色氨酸组HLA-A,B,C-PE染色的平均荧光强度分别为309.17±34.89及692.12±33.42,两者相比亦有显著性差异（P〈0.01）。结论：IDO下调细胞表面HLA-I类分子的表达,色氨酸能够逆转IDO所致的HLA-I类分子的下调。
英文摘要：
Aim： To investigate the effect of human IDO on the expression of cell surface MHC class I antigen. Methods： The 293T cells were transfected with pEGFP-N1 and pEGFP-IDO plasmids respectively by lipefectamine method. The expression of IDO in 293T cells transfected with recombinant plasraids was determined by Western blot. The expression of cell surface HI.,A-A, B, C molecules was tested in cells transfected with corresponding plasmids by flow cytometry analysis. Results： The IDO was expressed in 293T cells transfected with recombinant plasmid. The value of mean fluorescence intensity （MFI） for HLA-A,B,C staining were 299.46 ±62. 65 and 42. 51 ±8. 19 in cells transfected with GFP or IDO-EGFP, and the value were 309. 17 ±34. 89 and 692. 12 ±33.42 in IDOGFP transfected cells with or without tryptophan respectively. There are significant difference of MFI staining between the cells trans- fected with vector and with IDO-EGFP （P 〈 0. 01 ）, and in IDO-EGFP transfected cells with or without tryptophan （P 〈0. 01 ）. Conclusion： Human IDO down regulates the expression of cell surface HLA-I molecules and tryptophan can counteract this effect.
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