中文摘要：

含有重金属等污染物质的集约化养殖畜禽粪便是我国现代有机肥的主要肥源,其长期施用可能会影响耕地土壤微生物群落的结构组成和功能。以江苏省海安长期施用集约化养殖畜禽粪便和单施化肥试验地的耕层土壤为研究对象,采用BIOLOG微平板法,对比分析了单施化肥和集约化畜禽粪便处理土壤微生物群落功能多样性的差异,旨在探明长期施用集约化养殖畜禽粪便对土壤微生物群落功能的影响。结果表明,两种土壤中施用集约化畜禽粪便处理平均颜色变化率（AWCD）均高于单施化肥土壤。与单施化肥处理比较,施用畜禽粪便处理土壤微生物群落物种丰富度和优势度没有明显改变,但物种均匀度方面有明显提高。与施用化肥比较,施用畜禽粪便处理土壤微生物显著提高了对胺类、碳水化合物类和氨基酸类碳源的利用能力。主成分分析和对应分析结果显示,不同施肥方式显著改变了土壤微生物群落对碳源的利用方向和能力。综上所述,施用集约化畜禽粪便能改变土壤微生物群落功能多样性。

英文摘要：

Intensive livestock and poultry manure（ILPM） is one of the main sources of modern organic fertilizers in China, however, it contains much more heavy metals and other pollutants. Long-term application of ILPM may influence the structure and function of microbial community in cultivated soil. In order to understand the effects of long-term application of ILPM on soil microbial community function, we conducted an experiment using the BIOLOG micro plate method. All the soil samples were collected from two long-term experiment plots with ILPM or chemical fertilizer in Haian county of Jiangsu province. Results showed that the average well color development（AWCD） in soil with application of ILPM was higher than that of soil with chemical fertilizer in two kinds of plots. Compared with soil in application of chemical fertilizer treatment, soil microbial community species richness and dominance in treatments with IPLM did not change significantly, but species evenness significantly increased. The utilization ability of soil microbes on amine, carbohydrate and amino acid in treatments with IPLM was higher than that in treatments with chemical fertilizer. Results of principal component analysis and correspondence analysis also showed that the direction and ability of soil microbial community carbon resources utilization were significantly different between treatments with ILPM and with chemical fertilizer. Our results indicated that application of intensive livestock and poultry manure could change the functional diversity of soil microbial community.