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过表达细胞周期蛋白的Dl对成熟表皮细胞去分化为表皮干细胞的作用
  • ISSN号：1000-6834
  • 期刊名称：《中国应用生理学杂志》
  • 时间：0
  • 分类：R34[医药卫生—基础医学]
  • 作者机构：[1]天津医科大学,天津300070, [2]解放军总医院第一附属医院全军创伤修复与组织再生重点实验室, [3]解放军总医院第一附属医院肿瘤一科,北京100048
  • 相关基金：国家973计划资助项目（2012CB518105）; 国家自然科学基金面上项目（81571905,81121004,81230041,81171798）
作者： 赵阿龙[1,2], 叶明[3], 张翠萍[2], 马奎[2], 周云超[2], 谭志军[1,2], 付小兵[2]
关键词： CCND1, 去分化, 表皮干细胞, 成熟表皮细胞, cyclin D1, dedifferentiation, epidermal stem cells, mature skin cells
中文摘要：

目的：观察过表达细胞周期蛋白D1（CCND1）对成熟表皮细胞去分化为表皮干细胞的调控作用。方法：构建携带CCND1基因的真核表达载体PEGFP-N1-CCND1,将PEGFP-N1-CCND1转染人成熟表皮细胞,5 d后,倒置显微镜下观察细胞形态;细胞计数法观察细胞的增殖情况;免疫荧光法检测表皮干细胞标志抗原β1整合素和成熟表皮细胞标志抗原CK10的表达变化。结果：转染PEGFP-N1-CCND1后,细胞体积变小,核浆比例增大;细胞增殖较快,细胞数量比对照组增加了4倍（P〈0.01）;表型检测结果显示,转染PEGFP-N1-CCND1组,表达干细胞标志性蛋白β1整合素表达阳性,成熟表皮细胞标志性蛋白CK10表达阴性,而转染空载体组则相反。结论：CCND1过表达能够诱导成熟表皮细胞去分化为表皮干细胞。

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期刊信息
  • 《中国应用生理学杂志》
  • 北大核心期刊（2008版）
  • 主管单位:
  • 主办单位:中国生理学会 卫生学环境医学研究所 军事医学科学院
  • 主编：汪海
  • 地址：天津市大理道1号
  • 邮编：300050
  • 邮箱：tjzgyish@163.com
  • 电话：022-84655184
  • 国际标准刊号：ISSN：1000-6834
  • 国内统一刊号：ISSN：12-1339/R
  • 邮发代号:6-16
  • 获奖情况:
  • 国家印刷质量二等奖,国防科技期刊一等奖
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,荷兰文摘与引文数据库,美国生物医学检索系统,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2000版）
  • 被引量:9411